Liu Shang-Guo, Qin Xiu-Guang, Zhao Bao-Sheng, Qi Bo, Yao Wen-Jian, Wang Tian-Yun, Li Han-Chen, Wu Xiang-Nan
Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100;
Oncol Lett. 2013 May;5(5):1639-1642. doi: 10.3892/ol.2013.1251. Epub 2013 Mar 12.
The aim of this study was to explore the association of specific microRNAs (miRNAs) with the development of esophageal cancer (EC) and to identify new molecular markers for EC by analyzing the expression profiles of miRNAs in EC tissues. The expression profiles of miRNAs in paired EC and paracancerous normal tissues were detected and bioinformatically analyzed using miRNA assays. The outcomes were validated using real-time polymerase chain reaction. The miRNA assays revealed a total of 60 differentially expressed miRNAs in the EC tissues compared with those in the paracancerous normal tissues. Among them, 51 had doubled or more than doubled their expression levels and 9 had halved their expression levels. The most markedly upregulated miRNAs were hsa-miR-15a, hsa-miR-28-3p, hsa-miR-31, hsa-miR-99b, hsa-miR-101, hsa-miR-130a, hsa-miR-143, hsa-miR-196b, hsa-miR-200a, hsa-miR-210, hsa-miR-452 and hsa-miR-27a, whereas the most markedly downregulated miRNAs included hsa-miR-30b, hsa-miR-223, hsa-miR-454, hsa-miR-486, hsa-miR-574-3p and hsa-miR-126. Specific miRNA expression profiles exist in EC tissues and may serve as novel EC molecular markers.
本研究的目的是通过分析食管癌（EC）组织中微小RNA（miRNA）的表达谱，探讨特定miRNA与EC发生发展的关联，并鉴定EC新的分子标志物。采用miRNA检测方法检测并通过生物信息学分析配对的EC组织和癌旁正常组织中miRNA的表达谱。结果通过实时聚合酶链反应进行验证。miRNA检测显示，与癌旁正常组织相比，EC组织中共有60种差异表达的miRNA。其中，51种miRNA的表达水平增加了一倍或一倍以上，9种miRNA的表达水平减半。上调最明显的miRNA是hsa-miR-15a、hsa-miR-28-3p、hsa-miR-31、hsa-miR-99b、hsa-miR-101、hsa-miR-130a、hsa-miR-143、hsa-miR-196b、hsa-miR-200a、hsa-miR-210、hsa-miR-452和hsa-miR-27a，而下调最明显的miRNA包括hsa-miR-30b、hsa-miR-223、hsa-miR-454、hsa-miR-486、hsa-miR-574-3p和hsa-miR-126。EC组织中存在特定的miRNA表达谱，可能作为EC新的分子标志物。
