Department of Orthopaedics and Trauma, Peking University People's Hospital , Beijing , P. R. China.
Artif Cells Nanomed Biotechnol. 2014 Jun;42(3):217-21. doi: 10.3109/21691401.2013.800082. Epub 2013 Jun 13.
Outgrowth of collateral sprouts from axons is a natural process that arises during development of and regeneration in the peripheral nervous system. Our previous study showed that if there are enough distal endoneurial tubes into which the proximal regenerative axons can grow, one axon can support three to four collaterals, at most. Here, the proximal half tibial nerve was fixed to the distal stump and served as the donor nerve. The number of myelinated axons was calculated after 4 months. The ratio of distal regenerative myelinated axon number to proximal donor nerve axon number was 1.83 with the tibial function index and the nerve conduction velocities of - 48.6 ± 6.8 and 27.8 ± 5.3 m/s. The regenerated collaterals were isolated and observed to sprout from the node of Ranvier with almost the same features of normal fibers, but with different electrophysiological characteristics. This finding shows the evidence of one-axon trunk several-collateral model in peripheral nerve regeneration and suggests that such multi-collateral regeneration model may be useful in peripheral nerve reconstruction.
轴突侧支芽的生长是一个自然过程，它发生在周围神经系统的发育和再生过程中。我们之前的研究表明，如果有足够的远端神经内膜管供近端再生轴突生长，那么一根轴突最多可以支持三到四个侧支。在这里，将近端半胫骨神经固定在远端残端上，作为供体神经。4 个月后计算有髓轴突的数量。远端再生有髓轴突数与近端供体神经轴突数的比值为 1.83，胫神经功能指数和神经传导速度分别为-48.6±6.8 和 27.8±5.3 m/s。分离并观察到再生的侧支从郎飞结发出，具有与正常纤维几乎相同的特征，但具有不同的电生理特征。这一发现表明周围神经再生中存在单轴突多侧支模型的证据，并提示这种多侧支再生模型可能有助于周围神经重建。
