Clinical Microbiology and Infectious Diseases, NDCLS, University of Oxford, OX3 9DU, UK.
Plasmid. 2013 Sep;70(2):277-83. doi: 10.1016/j.plasmid.2013.05.008. Epub 2013 Jun 10.
A significant part of horizontal gene transfer is facilitated by genomic islands. Haemophilus influenzae genomic island ICEHin1056 is an archetype of a genomic island that accounts for pandemic spread of antibiotics resistance. ICEHin1056 has modular structure and harbors modules involved in type IV secretion and integration. Previous studies have shown that ICEHin1056 encodes a functional type IV secretion system; however, other modules have not been characterized yet. Here we show that the module on the 5' extremity of ICEHin1056 consists of 15 genes that are well conserved in a number of related genomic islands. Furthermore by disrupting six genes of the investigated module of ICEHin1056 by site-specific mutagenesis we demonstrate that in addition to type IV secretion system module, the investigated module is also important for the successful conjugal transfer of ICEHin1056 from donor to recipient cells.
水平基因转移的一个重要部分是由基因组岛促成的。流感嗜血杆菌基因组岛 ICEHin1056 是一种基因组岛的原型，它导致了抗生素耐药性的大流行传播。ICEHin1056 具有模块化结构，包含参与 IV 型分泌和整合的模块。先前的研究表明，ICEHin1056 编码了一个功能性的 IV 型分泌系统；然而，其他模块尚未得到表征。在这里，我们表明 ICEHin1056 5'端的模块由 15 个基因组成，这些基因在许多相关的基因组岛中高度保守。此外，通过对 ICEHin1056 中被研究模块的六个基因进行定点诱变，我们证明除了 IV 型分泌系统模块外，被研究的模块对于 ICEHin1056 从供体到受体细胞的成功接合转移也是重要的。
