Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242; CNRS/Université de Strasbourg, Illkirch, France.
MAbs. 2013 Jul-Aug;5(4):518-22. doi: 10.4161/mabs.25084. Epub 2013 May 29.
Antibodies are valuable tools for functional studies in vitro, but their use in living cells remains challenging because they do not naturally cross the cell membrane. Here, we present a simple and highly efficient method for the intracytoplasmic delivery of any antibody into cultured cells. By following the fate of monoclonal antibodies that bind to nuclear antigens, it was possible to image endogenous targets and to show that inhibitory antibodies are able to induce cell growth suppression or cell death. Our electrotransfer system allowed the cancer cells we studied to be transduced without loss of viability and may have applications for a variety of intracellular immuno-interventions.
抗体是体外功能研究的有价值工具，但由于它们不能自然穿过细胞膜，因此在活细胞中的应用仍然具有挑战性。在这里，我们提出了一种将任何抗体高效递送至培养细胞的胞质内的简单方法。通过跟踪结合核抗原的单克隆抗体的命运，我们可以对内源性靶标进行成像，并表明抑制性抗体能够诱导细胞生长抑制或细胞死亡。我们的电转移系统允许我们研究的癌细胞在不失活的情况下被转导，并且可能适用于各种细胞内免疫干预。
