Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Cell Biol Int. 2013 Nov;37(11):1181-9. doi: 10.1002/cbin.10143. Epub 2013 Jul 8.
Bone matrix consists of two major phases at the nanoscale: organic and hydroxyapatite. Nanotechnology as a diverse and interdisciplinary area of research has the capacity to revolutionise many areas of applications such as bone tissue engineering. Nanohydroxyapatite/gelatin composite has higher osteoblast attachment and proliferation than micro-sized ones, and shorter culturing period and lower cell seeding density compared to pure gelatin. A nanostructured scaffold was fabricated by three methods for bone repair using nanohydroxyapatite and gelatin as the main components. Its biocompatibility, alizarin red test on the 14th and 21st days, gene expression on the 21st day in in vitro using and histomorphometry after 4 and 8 weeks post-implantation in the rat were investigated. Cultured unrestricted somatic stem cells used for in vitro study showed an excellent level of cell attachment to the scaffold. Cells induced more osteoblast differentiation on the scaffold than in 2D cell culture. Osteoblast differentiation and bone regeneration results of in vitro and in vivo investigation on scaffold were extremely significant, better than control and treatment groups. These effects could be attributed to the shape and size of nanoHA particles and good architecture of the scaffold. The results confirm the feasibility of bone regeneration using synthesised scaffold as a temporary bone substitute.
有机相和羟磷灰石相。纳米技术作为一个多样化和跨学科的研究领域，有能力彻底改变许多应用领域，如骨组织工程。纳米羟基磷灰石/明胶复合材料的成骨细胞附着和增殖能力高于微尺寸的，培养周期短，细胞接种密度低于纯明胶。采用三种方法制备了一种以纳米羟基磷灰石和明胶为主要成分的用于骨修复的纳米结构支架。对其在体外的生物相容性、第 14 天和第 21 天的茜素红试验、第 21 天的基因表达以及植入大鼠 4 周和 8 周后的组织形态计量学进行了研究。用于体外研究的培养无限制的体干细胞对支架表现出极好的细胞附着水平。与 2D 细胞培养相比，细胞在支架上诱导出更多的成骨细胞分化。支架的体外和体内骨再生结果非常显著，优于对照组和治疗组。这些效果可能归因于纳米 HA 颗粒的形状和尺寸以及支架的良好结构。研究结果证实了使用合成支架作为临时骨替代物进行骨再生的可行性。
