从天蓝色链霉菌（天青菌素的产生菌）中克隆林可酰胺类抗性决定簇并对抗性机制进行表征。
Cloning of a lincosamide resistance determinant from Streptomyces caelestis, the producer of celesticetin, and characterization of the resistance mechanism.
作者信息
Calcutt M J, Cundliffe E
机构信息
Department of Biochemistry, University of Leicester, England.
出版信息
J Bacteriol. 1990 Aug;172(8):4710-4. doi: 10.1128/jb.172.8.4710-4714.1990.
Abstract
Self-resistance has been investigated in Streptomyces caelestis (producer of the lincosamide antibiotic celesticetin), from which a lincosamide resistance determinant (clr) has been isolated on a 1-kilobase DNA fragment and cloned in Streptomyces lividans. The clr product is a specific methylase which produces a single residue of N6-monomethyladenine in 23S rRNA at position 2058, thereby rendering the 50S ribosmal subunit resistant to the action of lincosamides.
摘要
已对天蓝色链霉菌（林可酰胺类抗生素天青菌素的产生菌）的自身抗性进行了研究，从该菌中在一个1千碱基的DNA片段上分离出一种林可酰胺抗性决定子（clr），并将其克隆到淡紫灰链霉菌中。clr产物是一种特异性甲基化酶，它在23S rRNA的2058位产生一个N6-单甲基腺嘌呤残基，从而使50S核糖体亚基对林可酰胺类的作用产生抗性。
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