Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK. Department of Bioresources, A-2444 Seibersdorf, Austria.
Environ Microbiol Rep. 2009 Oct;1(5):398-407. doi: 10.1111/j.1758-2229.2009.00059.x. Epub 2009 Aug 13.
Methanotrophs present in landfill cover soil can limit methane emissions from landfill sites by oxidizing methane produced in landfill. Understanding the spatial and temporal distribution of populations of methanotrophs and the factors influencing their activity and diversity in landfill cover soil is critical to devise better landfill cover soil management strategies. pmoA-based microarray analyses of methanotroph community structure revealed a temporal shift in methanotroph populations across different seasons. Type II methanotrophs (particularly Methylocystis sp.) were found to be present across all seasons. Minor shifts in type I methanotroph populations were observed. In the case of spatial distribution, only minor differences in methanotroph community structure were observed with no recognizable patterns (both vertical and horizontal) at a 5 m scale. Correlation analysis between soil abiotic parameters (total C, N, NH4 (+) , NO3 (-) and water content) and distribution of methanotrophs revealed a lack of conclusive evidence for any distinct correlation pattern between measured abiotic parameters and methanotroph community structure, suggesting that complex interactions of several physico-chemical parameters shape methanotroph diversity and activity in landfill cover soils.
在垃圾填埋场封场土壤中存在的产甲烷菌可以通过氧化垃圾填埋场产生的甲烷来限制甲烷排放。了解封场土壤中产甲烷菌的时空分布以及影响其活性和多样性的因素，对于制定更好的封场土壤管理策略至关重要。基于 pmoA 的微阵列分析表明，不同季节中产甲烷菌群落结构发生了时间上的转变。发现所有季节都存在 II 型产甲烷菌（特别是甲基球菌属）。I 型产甲烷菌的种群略有变化。在空间分布方面，在 5 米的尺度上，仅观察到产甲烷菌群落结构的微小差异，没有明显的模式（垂直和水平）。对土壤非生物参数（总 C、N、NH4 (+) 、NO3 (-) 和含水量）与产甲烷菌分布之间的相关性分析表明，在测量的非生物参数与产甲烷菌群落结构之间缺乏明确的相关模式的结论性证据，这表明几种物理化学参数的复杂相互作用塑造了封场土壤中产甲烷菌的多样性和活性。
