Alcântara D D F A, Ribeiro H F, Matos L A, Sousa J M C, Burbano R R, Bahia M O
Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
Genet Mol Res. 2013 May 13;12(2):1554-60. doi: 10.4238/2013.May.13.9.
Iron is the most important metallic chemical element on Earth. Poisoning caused by excessive iron in humans has been associated with pulmonary diseases including neoplasms caused by inhalation of iron oxides. The involvement of iron in neurodegenerative processes has already been described. DNA alterations are induced by iron and other chemical compounds containing this metal; however, the data are controversial and the mechanism by which iron induces mutagenesis remains unknown. This study assessed in vitro iron-induced cytotoxic and genotoxic responses in an astrocytic cell line. Short- and long-term cytotoxicity and genotoxicity were evaluated with the Cell Proliferation Kit II and micronucleus test, respectively. Results indicated that the highest concentration of iron sulfate tested was cytotoxic in long-term cytotoxic assays and increased micronucleus frequency in comparison to controls. The significant cytotoxicity observed here might be due to the intrinsic ability of iron to induce apoptosis and possible changes in cell cycle kinetics; the genotoxic effects are probably due to the oxidant properties of iron itself. This was the first study to investigate the induction of micronuclei by iron in central nervous system cells.
铁是地球上最重要的金属化学元素。人体中铁过量导致的中毒与肺部疾病有关，包括吸入氧化铁引发的肿瘤。铁在神经退行性过程中的作用已有描述。铁和其他含该金属的化合物可诱导DNA改变；然而，数据存在争议，铁诱导诱变的机制仍不清楚。本研究评估了铁在体外对星形胶质细胞系的细胞毒性和遗传毒性反应。分别使用细胞增殖试剂盒II和微核试验评估短期和长期细胞毒性及遗传毒性。结果表明，所测试的最高浓度硫酸铁在长期细胞毒性试验中具有细胞毒性，与对照组相比，微核频率增加。此处观察到的显著细胞毒性可能归因于铁诱导细胞凋亡的内在能力以及细胞周期动力学的可能变化；遗传毒性效应可能是由于铁本身的氧化特性。这是第一项研究铁在中枢神经系统细胞中诱导微核的研究。
