Terpiłowska Sylwia, Siwicka-Gieroba Dorota, Siwicki Andrzej Krzysztof
Laboratory of Environmental Biology, Institute of Environmental Engineering, The John Paul II Catholic University of Lublin, 20-950 Lublin, Poland.
Department of Anaesthesiology and Intensive Care, Faculty of Medicine, Medical University in Lublin, 20-850 Lublin, Poland.
J Vet Res. 2018 Dec 31;62(4):527-533. doi: 10.2478/jvetres-2018-0066. eCollection 2018 Dec.
Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA.
BALB/3T3 and HepG2 cells were incubated with iron chloride or molybdenum trioxide at concentrations from 100 to 1,400 μM. The cells were also incubated in mixtures of iron chloride at 200 μM plus molybdenum trioxide at 1,000 μM or iron chloride at 1,000 μM plus molybdenum trioxide at 200 μM. Cell viability was determined with MTT reduction, LHD release, and NRU tests.
A decrease in cell viability was observed after incubating both cell lines with iron chloride or molybdenum trioxide. In cells incubated with mixtures of these trace elements, a decrease in cell viability was observed, assessed by all the used assays.
Iron (III) and molybdenum (III) decrease cell viability in normal and cancer cells. A synergistic effect of the mixture of these elements was observed.
铁和钼是细胞代谢所必需的微量元素。它们参与维持酶的正常功能、细胞增殖以及DNA代谢。
将BALB/3T3细胞和HepG2细胞与浓度为100至1400μM的氯化铁或三氧化钼一起孵育。细胞还分别在200μM氯化铁加1000μM三氧化钼或1000μM氯化铁加200μM三氧化钼的混合物中孵育。通过MTT还原法、LHD释放法和NRU试验测定细胞活力。
用氯化铁或三氧化钼孵育这两种细胞系后，观察到细胞活力下降。在用这些微量元素混合物孵育的细胞中，通过所有使用的检测方法评估，均观察到细胞活力下降。
铁（III）和钼（III）会降低正常细胞和癌细胞的活力。观察到这些元素混合物具有协同作用。
