阻塞性睡眠呼吸暂停患者外周血中性粒细胞中活性氧的产生
Reactive oxygen species production in peripheral blood neutrophils of obstructive sleep apnea patients.
作者信息
Pilkauskaite Guoda, Miliauskas Skaidrius, Sakalauskas Raimundas
机构信息
Department of Pulmonology and Immunology, Medical Academy, Lithuanian University of Health Sciences, Eiveniu 2, 50009 Kaunas, Lithuania.
出版信息
ScientificWorldJournal. 2013 May 12;2013:421763. doi: 10.1155/2013/421763. Print 2013.
UNLABELLED
Obstructive sleep apnea (OSA) as well as obesity is associated with increased production of reactive oxygen species (ROS). Neutrophils produce great amounts of ROS. The AIM was to evaluate peripheral blood neutrophils ROS production in men with OSA and to establish relations with disease severity and obesity.
METHODS
Forty-six men with OSA and 10 controls were investigated. OSA was confirmed by polysomnography (PSG), when apnea/hypopnea index was >5/h. Body mass index (BMI) was evaluated. Neutrophils were isolated from peripheral blood in the morning after PSG. Dihydrorhodamine-123 was used for ROS detection. Data is presented as median (25th and 75th percentiles). All subjects were divided into four groups: nonobese mild-to-moderate OSA, obese mild-to-moderate OSA, nonobese severe OSA, and obese severe OSA.
RESULTS
Neutrophil ROS production was higher in nonobese severe OSA group compared to nonobese mild-to-moderate OSA (mean fluorescence intensity (MFI) 213.4 (89.0-238.9) versus 44.5 (20.5-58.4), P < 0.05). In obese patient groups, ROS production was more increased in severe OSA compared to mild-to-moderate OSA group (MFI 74.5 (47.9-182.4) versus 31.0 (14.8-53.8), P < 0.05). It did not differ in the groups with different BMI and the same severity of OSA.
CONCLUSION
Increased neutrophil ROS production was related to more severe OSA but not obesity.
未标注
阻塞性睡眠呼吸暂停（OSA）以及肥胖与活性氧（ROS）生成增加有关。中性粒细胞会产生大量ROS。本研究旨在评估男性阻塞性睡眠呼吸暂停患者外周血中性粒细胞ROS的生成情况，并确定其与疾病严重程度和肥胖之间的关系。
方法
对46例阻塞性睡眠呼吸暂停男性患者和10例对照者进行研究。通过多导睡眠图（PSG）确诊阻塞性睡眠呼吸暂停，呼吸暂停/低通气指数>5次/小时。评估体重指数（BMI）。在PSG检查后的早晨从外周血中分离中性粒细胞。使用二氢罗丹明-123检测ROS。数据以中位数（第25和第75百分位数）表示。所有受试者分为四组：非肥胖轻度至中度OSA组、肥胖轻度至中度OSA组、非肥胖重度OSA组和肥胖重度OSA组。
结果
与非肥胖轻度至中度OSA组相比，非肥胖重度OSA组中性粒细胞ROS生成更高（平均荧光强度（MFI）分别为213.4（89.0 - 238.9）和44.5（20.5 - 58.4），P < 0.05）。在肥胖患者组中，重度OSA组的ROS生成比轻度至中度OSA组增加更多（MFI分别为74.5（47.9 - 182.4）和31.0（14.8 - 53.8），P < 0.05）。在不同BMI且OSA严重程度相同的组之间没有差异。
结论
中性粒细胞ROS生成增加与更严重的OSA有关，而与肥胖无关。
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