Revencu Tatiana, Trifan Victoria, Nacu Ludmila, Gutium Tatiana, Globa L, Motoc A G M, Nacu V
Laboratory of Tissue Engineering and Cell Cultures, Department of Obstetrics and Gynecology, "Nicolae Testemitanu" State Medical and Pharmaceutical University, Kishinev, Republic of Moldova.
Rom J Morphol Embryol. 2013;54(2):291-7.
The umbilical cord blood (UCB) is a rich source of hematopoietic and mesenchymal stem cells and it can be used for cellular therapy. This article presents data achieved from 50 cord blood samples (46 in utero, four ex utero). The nucleated cells were isolated from the cord blood using a dextran solution. The quantity of stem cells having the immunophenotype CD34+, CD45+ and CD90+ was determined, using flow cytometry. The nucleated cells were cultivated in a DMEM (Dulbecco's Modified Eagle Medium) and biochemical and cytogenetic tests was later performed.
脐带血是造血干细胞和间充质干细胞的丰富来源，可用于细胞治疗。本文展示了从50份脐带血样本（46份宫内样本，4份宫外样本）中获得的数据。使用葡聚糖溶液从脐带血中分离有核细胞。采用流式细胞术测定具有免疫表型CD34+、CD45+和CD90+的干细胞数量。将有核细胞在杜氏改良伊格尔培养基（DMEM）中培养，随后进行生化和细胞遗传学检测。
