Graduate Institute of Marine Biotechnology and Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan.
Mar Drugs. 2013 Jun 17;11(6):2154-67. doi: 10.3390/md11062154.
A new norcembranoidal diterpene, 1-epi-sinulanorcembranolide A (1), and a new cembranoidal diterpene, flexibilin D (2), were isolated from the soft corals, Sinularia gaweli and Sinularia flexibilis, respectively. The structures of new metabolites 1 and 2 were elucidated by spectroscopic methods, and compound 2 was found to significantly inhibit the accumulation of the pro-inflammatory iNOS and COX-2 proteins of the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. In addition, S. flexibilis yielded a known cembrane, 5-dehydrosinulariolide (3); the structure, including its absolute stereochemistry, was further confirmed by single-crystal X-ray diffraction analysis.
从软珊瑚 Sinularia gaweli 和 Sinularia flexibilis 中分别分离得到一个新的诺卡烯二萜 1-表-辛拉诺醇内酯 A（1）和一个新的海鞘二萜 flexibilin D（2）。通过光谱方法阐明了新代谢物 1 和 2 的结构，并且发现化合物 2 显著抑制脂多糖（LPS）刺激的 RAW264.7 巨噬细胞中促炎 iNOS 和 COX-2 蛋白的积累。此外，S. flexibilis 产生了一种已知的海鞘烯，5-脱水辛拉醇内酯（3）；其结构，包括其绝对立体化学，通过单晶 X 射线衍射分析进一步证实。
