National Museum of Marine Biology & Aquarium, Pingtung 944,Taiwan.
Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944,Taiwan.
Mar Drugs. 2011;9(6):944-951. doi: 10.3390/md9060944. Epub 2011 May 27.
Chemical examination of the Taiwanese soft coral Sinularia triangular led to the isolation of five cembrane-based diterpenoids 1-5, including two new metabolites, triangulenes A (1) and B (2). The structures of the new metabolites were determined on the basis of extensive spectroscopic analysis, particularly mass spectroscopy and 2D NMR ((1)H-(1)H COSY, HMQC, HMBC, and NOESY) spectroscopy. Metabolites 3 and 5 exhibited moderate cytotoxicity to human tumor cell lines CCRF-CEM and DLD-1. Furthermore, 3-5 displayed significant in vitro anti-inflammatory activity in lipopolysaccharide-stimulated RAW264.7 macrophage cells by inhibiting the expression of the iNOS protein. Metabolites 4 and 5 also effectively reduced the expression of the COX-2 protein in the macrophages.
对台湾软珊瑚 Sinularia triangular 的化学研究导致了五种基于cembrane 的二萜类化合物 1-5 的分离，包括两种新的代谢物，三角烯 A（1）和 B（2）。新代谢物的结构是根据广泛的光谱分析确定的，特别是质谱和二维 NMR ((1)H-(1)H COSY、HMQC、HMBC 和 NOESY) 光谱。代谢物 3 和 5 对人肿瘤细胞系 CCRF-CEM 和 DLD-1 表现出中等的细胞毒性。此外，3-5 通过抑制 iNOS 蛋白的表达，在脂多糖刺激的 RAW264.7 巨噬细胞中表现出显著的体外抗炎活性。代谢物 4 和 5 也能有效降低巨噬细胞中 COX-2 蛋白的表达。
