Organic and Biomimetic Chemistry Research Group, Department of Organic Chemistry, Faculty of Sciences, Ghent University, Krijgslaan 281 (S4), 9000 Ghent, Belgium.
Chembiochem. 2013 Jun 17;14(9):1032-48. doi: 10.1002/cbic.201200775.
With the study of peptides and proteins at the heart of many scientific endeavors, the omics era heralded a multitude of opportunities for chemists and biologists alike. Across the interface with life sciences, peptide chemistry plays an indispensable role, and progress made over the past decades now allows proteins to be treated as molecular patchworks stitched together through synthetic tailoring. The continuous elaboration of sophisticated strategies notwithstanding, Merrifield's solid-phase methodology remains a cornerstone of chemical protein design. Although the non-practitioner might misjudge peptide synthesis as trivial, routine, or dull given its long history, we comment here on its many advances, obstacles, and prospects from a practitioner's point of view. While sharing our perspectives through thematic highlights across the literature, this treatise provides an interpretive overview as a guide to novices, and a recap for specialists.
在许多科学研究中，肽和蛋白质的研究都是核心内容，组学时代为化学家们带来了大量的机会。在与生命科学的交叉界面上，肽化学起着不可或缺的作用，过去几十年的进展使得蛋白质可以被视为通过合成剪裁拼接在一起的分子拼贴画。尽管不断完善复杂的策略，但 Merrifield 的固相方法仍然是化学蛋白质设计的基石。尽管非从业者可能会因其悠久的历史而低估肽合成的重要性、常规性或枯燥性，但我们在此从从业者的角度评论其许多进展、障碍和前景。虽然我们通过文献中的主题重点分享我们的观点，但本文作为新手的指南和专家的复习，提供了一个解释性的概述。
