School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.
School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.
Curr Opin Chem Biol. 2014 Oct;22:70-8. doi: 10.1016/j.cbpa.2014.09.021. Epub 2014 Oct 3.
Native chemical ligation continues to play a pivotal role in the synthesis of increasingly complex peptide and protein targets twenty years after its initial report. This opinion article will highlight a number of recent, powerful extensions of the technology that have expanded the scope of the reaction, accelerated ligation rates, enabled chemoselective post-ligation modifications, and streamlined the ligation of multiple peptide fragments. These advances have facilitated the synthesis of a number of impressive protein targets to date and hold great promise for the continued application of native chemical ligation for the detailed study of protein structure and function.
天然化学连接在其最初报道二十年后，在合成越来越复杂的肽和蛋白质靶标方面继续发挥着关键作用。本文将重点介绍该技术的一些最新、强大的扩展，这些扩展扩大了反应的范围，加快了连接速度，实现了化学选择性的连接后修饰，并简化了多个肽片段的连接。这些进展促进了许多令人印象深刻的蛋白质靶标的合成，并为天然化学连接在蛋白质结构和功能的详细研究中的持续应用提供了巨大的前景。
