Sub-Department of Molecular Genetics, Institute of Public Health, Santiago, Chile.
PLoS One. 2013 Jun 11;8(6):e66006. doi: 10.1371/journal.pone.0066006. Print 2013.
With the upcoming licensure of Outer Membrane Protein-based vaccines against meningococcal disease, data on disease incidence and molecular characteristic of circulating N. meningitidis strains in Latin American countries is needed. Chile is, to date, one of the few countries in the region that has performed this type of work in a comprehensive collection of disease-associated strains from two consecutive years, 2010-2011.
A total of 119 N. meningitidis strains isolated from patients with invasive disease in Chile in 2010-2011 were characterized by the National Reference Laboratory. Serogroup determination, MLST and porA typing were performed.
Serogroup B was predominant in both study years, but W135 experienced a noticeable increase in 2011 compared to 2010. ST-11 complex, ST-41/44 complex ST-32 complex were the most prevalent among the isolates, and were strongly associated with serogroups W135 (ST-11 Complex) and B (ST-41/44 and ST-32 complexes). Likewise, the major porA types detected were strongly associated with these three clonal complexes: P1.5,2 was found exclusively among W135:ST-11 isolates, whereas P1.7, 2-3 was only detected in C:ST-11. ST-41/44 isolates mainly had P1.10-8, and ST-32 complex were associated with a P1.18-8 porA.
Our data show disease-associated N. meningitidis circulating in Chile are similar to those found in other parts of the world. The increase on W135:ST-11 isolates observed in 2011 foretold the unusual epidemiological situation experienced in the country in 2012, and MLST data show that this strain is indistinguishable from the one linked to the global Hajj 2000-related outbreak that occurred in 2001. Finally, this work demonstrates the importance of maintaining a strong national surveillance program integrating clinical, epidemiological and laboratory data and incorporating gold standard diagnostic and characterization techniques that allow the data to be compared all over the world.
随着外膜蛋白疫苗对抗脑膜炎奈瑟菌疾病的即将许可，拉丁美洲国家需要有关疾病发病率和循环脑膜炎奈瑟菌菌株分子特征的数据。智利是迄今为止该地区少数几个在两年连续时间内对与疾病相关的菌株进行全面收集并开展此类工作的国家之一，时间范围为 2010 年至 2011 年。
2010-2011 年，智利国家参考实验室对从侵袭性疾病患者中分离的 119 株脑膜炎奈瑟菌进行了特征描述。进行了血清群确定、MLST 和 porA 分型。
在这两年中，血清群 B 均占主导地位，但与 2010 年相比，2011 年 W135 的比例明显增加。ST-11 复合群、ST-41/44 复合群和 ST-32 复合群是分离株中最常见的复合群，与血清群 W135（ST-11 复合群）和 B（ST-41/44 和 ST-32 复合群）密切相关。同样，检测到的主要 porA 类型也与这三个克隆复合群密切相关：P1.5,2 仅存在于 W135：ST-11 分离株中，而 P1.7,2-3 仅存在于 C：ST-11 中。ST-41/44 分离株主要具有 P1.10-8，而 ST-32 复合群与 P1.18-8 porA 相关。
我们的数据表明，在智利流行的与疾病相关的脑膜炎奈瑟菌与世界其他地区发现的脑膜炎奈瑟菌相似。2011 年观察到 W135：ST-11 分离株的增加预示着该国在 2012 年经历了不寻常的流行病学情况，MLST 数据表明，这种菌株与 2001 年发生的与全球朝觐 2000 相关的全球暴发有关的菌株无法区分。最后，这项工作表明，必须维持一个强大的国家监测计划，该计划整合临床、流行病学和实验室数据，并采用金标准诊断和特征描述技术，以便能够在全球范围内比较数据。
