Centre for Molecular Microbiology and Infection, Department of Microbiology, Imperial College London, London, United Kingdom.
PLoS One. 2010 Feb 25;5(2):e9413. doi: 10.1371/journal.pone.0009413.
Previously we have shown that insertion of IS1301 in the sia/ctr intergenic region (IGR) of serogroup C Neisseria meningitidis (MenC) isolates from Spain confers increased resistance against complement-mediated killing. Here we investigate the significance of IS1301 in the same location in N. meningitidis isolates from the UK. PCR and sequencing was used to screen a collection of more than 1500 meningococcal carriage and disease isolates from the UK for the presence of IS1301 in the IGR. IS1301 was not identified in the IGR among vaccine failure strains but was frequently found in serogroup B isolates (MenB) from clonal complex 269 (cc269). Almost all IS1301 insertions in cc269 were associated with novel polymorphisms, and did not change capsule expression or resistance to human complement. After excluding sequence types (STs) distant from the central genotype within cc269, there was no significant difference for the presence of IS1301 in the IGR of carriage isolates compared to disease isolates. Isolates with insertion of IS1301 in the IGR are not responsible for MenC disease in UK vaccine failures. Novel polymorphisms associated with IS1301 in the IGR of UK MenB isolates do not lead to the resistance phenotype seen for IS1301 in the IGR of MenC isolates.
先前我们已表明，插入物 IS1301 在西班牙血清群 C 脑膜炎奈瑟菌（MenC）分离株的 sia/ctr 基因间区（IGR）中，赋予了对补体介导杀伤的增加抗性。在此，我们研究了 IS1301 在英国相同位置的脑膜炎奈瑟菌分离株中的意义。PCR 和测序用于筛选来自英国的超过 1500 例脑膜炎球菌携带和疾病分离株集合，以检测 IGR 中 IS1301 的存在。IS1301 在疫苗失败株的 IGR 中未被识别，但在克隆复合物 269（cc269）的血清群 B 分离株（MenB）中频繁发现。cc269 中的几乎所有 IS1301 插入均与新的多态性相关，并且不改变荚膜表达或对人补体的抗性。在排除与 cc269 内中心基因型相距较远的序列型（ST）之后，与疾病分离株相比，携带分离株的 IGR 中 IS1301 的存在没有显著差异。在 IGR 中插入 IS1301 的分离株与英国疫苗失败的 MenC 疾病无关。与 IS1301 在英国 MenB 分离株的 IGR 相关的新多态性不会导致与 IS1301 在 IGR 中看到的 MenC 分离株的抗性表型。
