Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA.
Environ Res. 2013 Oct;126:159-63. doi: 10.1016/j.envres.2013.05.007. Epub 2013 Jun 15.
1,2-Cyclohexane dicarboxylic acid, diisononyl ester (DINCH) is a complex mixture of nine carbon branched-chain isomers. It has been used in Europe since 2002 as a plasticizer to replace phthalates such as di(2-ethylhexyl)phthalate (DEHP) and diisononyl phthalate (DINP). Urinary concentrations of the oxidative metabolites of DINCH, namely cyclohexane-1,2-dicarboxylic acid-monocarboxy isooctyl ester (MCOCH); cyclohexane-1,2-dicarboxylic acid-mono(oxo-isononyl) ester (MONCH); and cyclohexane-1,2-dicarboxylic acid-mono(hydroxy-isononyl) ester (MHNCH), can potentially be used as DINCH exposure biomarkers. The concentrations of MCOCH, MONCH and MHNCH were measured by online solid phase extraction-high performance liquid chromatography-tandem mass spectrometry in urine collected in 2000 (n=114), 2001 (n=57), 2007 (n=23), 2009 (n=118), 2011 (n=94) and 2012 (n=121) from convenience groups of anonymous U.S. adult volunteers with no known DINCH exposure. None of the DINCH metabolites were detected in samples collected in 2000 and 2001. Only one sample collected in 2007 had measureable concentrations of DINCH metabolites. The detection rate for all three metabolites increased from 2007 to 2012. The presence of oxidative metabolites of DINCH in urine suggests that these oxidative metabolites can be used as DINCH biomarkers for exposure assessment even at environmental exposure levels.
1,2-环己烷二羧酸，二异壬酯（DINCH）是一种复杂的九碳支链异构体混合物。自 2002 年以来，它已在欧洲被用作增塑剂，以替代邻苯二甲酸二（2-乙基己基）酯（DEHP）和邻苯二甲酸二异壬酯（DINP）等邻苯二甲酸酯。DINCH 的氧化代谢物，即环己烷-1,2-二羧酸-单羧酸异壬酯（MCOCH）；环己烷-1,2-二羧酸-单（氧代-异壬基）酯（MONCH）；和环己烷-1,2-二羧酸-单（羟基-异壬基）酯（MHNCH），可作为 DINCH 暴露生物标志物。MCOCH、MONCH 和 MHNCH 的浓度通过在线固相萃取-高效液相色谱-串联质谱法在 2000 年（n=114）、2001 年（n=57）、2007 年（n=23）、2009 年（n=118）、2011 年（n=94）和 2012 年（n=121）收集的尿液中进行了测量，这些尿液来自于美国匿名成年志愿者的便利组，他们没有已知的 DINCH 暴露。在 2000 年和 2001 年收集的样本中均未检测到任何 DINCH 代谢物。2007 年只有一个样本可检测到 DINCH 代谢物的浓度。2007 年至 2012 年，所有三种代谢物的检出率均有所增加。尿液中存在 DINCH 的氧化代谢物表明，即使在环境暴露水平下，这些氧化代谢物也可作为 DINCH 暴露评估的生物标志物。
