Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil.
Anal Bioanal Chem. 2013 Sep;405(24):7615-42. doi: 10.1007/s00216-013-7077-9. Epub 2013 Jun 19.
Fluorine, chlorine, bromine, and iodine have been studied in biological samples and other related matrices owing to the need to understand the biochemical effects in living organisms. In this review, the works published in last 20 years are covered, and the main topics related to sample preparation methods and analytical techniques commonly used for fluorine, chlorine, bromine, and iodine determination in biological samples, food, drugs, and plants used as food or with medical applications are discussed. The commonest sample preparation methods, as extraction and decomposition using combustion and pyrohydrolysis, are reviewed, as well as spectrometric and electroanalytical techniques, spectrophotometry, total reflection X-ray fluorescence, neutron activation analysis, and separation systems using chromatography and electrophoresis. On this aspect, the main analytical challenges and drawbacks are highlighted. A discussion related to the availability of certified reference materials for evaluation of accuracy is also included, as well as a discussion of the official methods used as references for the determination of halogens in the samples covered in this review.
氟、氯、溴、碘已在生物样本和其他相关基质中进行了研究，因为需要了解生物体内的生化效应。本综述涵盖了过去 20 年发表的研究工作，讨论了用于生物样本、食品、药物以及用作食品或具有医学应用的植物中氟、氯、溴和碘测定的常用样品制备方法和分析技术的主要主题。综述了最常用的样品制备方法，如使用燃烧和热解进行提取和分解，以及光谱和电化学分析技术、分光光度法、全反射 X 射线荧光法、中子激活分析以及使用色谱和电泳的分离系统。在这方面，突出了主要的分析挑战和缺点。还讨论了评估准确性的认证参考物质的可用性，以及作为本综述所涵盖样品中卤素测定参考的官方方法的讨论。
