Division of Endocrinology and Metabolism, Department of Medicine, University of Texas Medical Branch, Galveston, Texas 77555-1060, USA.
J Clin Endocrinol Metab. 2013 Aug;98(8):E1360-3. doi: 10.1210/jc.2012-3841. Epub 2013 Jun 18.
Obesity is thought to be the driving force for activation of adipose tissue (AT) collagen production and inflammation as well as systemic insulin resistance. The objective of this study was to determine whether these AT abnormalities can be found independent of obesity in the presence of systemic insulin resistance.
Thirty-eight normoglycemic men (14 Asian Indians and 24 white) were enrolled in the study and matched for age, body mass index, and total body fat. Subjects underwent anthropometric measurement, total body fat determination by underwater weighing, euglycemic-hyperinsulinemic clamps, and abdominal sc AT biopsy for mRNA extraction and gene expression determination. Fasting blood was collected for adipokine measurements.
Both groups were matched for age, body mass index, and percentage of total body fat. Subcutaneous abdominal AT mRNA expression was significantly higher for Col6a3 as well as genes associated with inflammation, CD68, MAC1, and MCP1 in Asian Indians compared with whites. Asian Indian men had significantly lower rates of glucose disposal and lower plasma adiponectin concentration. Plasma high-sensitivity C-reactive protein levels showed a trend towards higher levels in Asian Indian men.
Increased col6a3 and macrophage infiltration in AT along with increased systemic insulin resistance is present independent of body fat content in young Asian Indian men, thus suggesting that AT dysfunction associates with systemic insulin resistance regardless of AT mass.
肥胖被认为是脂肪组织（AT）胶原产生和炎症以及全身胰岛素抵抗激活的驱动力。本研究的目的是确定在存在全身胰岛素抵抗的情况下，这些 AT 异常是否可以独立于肥胖而存在。
研究纳入了 38 名血糖正常的男性（14 名印度裔和 24 名白人），并根据年龄、体重指数和全身脂肪量进行匹配。受试者接受了人体测量、水下称重确定全身脂肪量、血糖正常高胰岛素钳夹和腹部皮下 AT 活检以提取 mRNA 并确定基因表达。采集空腹血进行脂肪因子测量。
两组在年龄、体重指数和全身脂肪百分比方面均匹配。与白人相比，印度裔男性的 Col6a3 以及与炎症相关的基因 CD68、MAC1 和 MCP1 的皮下腹部 AT mRNA 表达明显更高。印度裔男性的葡萄糖摄取率明显较低，血浆脂联素浓度也较低。血浆高敏 C 反应蛋白水平在印度裔男性中呈升高趋势。
年轻的印度裔男性中存在 Col6a3 增加和 AT 巨噬细胞浸润以及全身胰岛素抵抗增加，这表明无论 AT 质量如何，AT 功能障碍都与全身胰岛素抵抗有关。
