Dowal Louisa, Parameswaran Pooja, Phat Sarah, Akella Syamala, Majumdar Ishita Deb, Ranjan Jyoti, Shah Chahan, Mogre Saie, Guntur Kalyani, Thapa Khampaseuth, Gesta Stephane, Vishnudas Vivek K, Narain Niven R, Sarangarajan Rangaprasad
Berg LLC, Framingham, MA, USA.
Mediators Inflamm. 2017;2017:9067049. doi: 10.1155/2017/9067049. Epub 2017 Mar 26.
Obesity is marked by chronic, low-grade inflammation. Here, we examined whether intrinsic differences between white and brown adipocytes influence the inflammatory status of macrophages. White and brown adipocytes were characterized by transcriptional regulation of , , , and and their level of IL-6 secretion. The inflammatory profile of PMA-differentiated U937 and THP-1 macrophages, in resting state and after stimulation with LPS/IFN-gamma and IL-4, was assessed by measuring IL-6 secretion and transcriptional regulation of a panel of inflammatory genes after mono- or indirect coculture with white and brown adipocytes. White adipocyte monocultures show increased IL-6 secretion compared to brown adipocytes. White adipocytes cocultured with U937 and THP-1 macrophages induced a greater increase in IL-6 secretion compared to brown adipocytes cocultured with both macrophages. White adipocytes cocultured with macrophages increased inflammatory gene expression in both types. In contrast, macrophages cocultured with brown adipocytes induced downregulation or no alterations in inflammatory gene expression. The effects of adipocytes on macrophages appear to be independent of stimulation state. Brown adipocytes exhibit an intrinsic ability to dampen inflammatory profile of macrophages, while white adipocytes enhance it. These data suggest that brown adipocytes may be less prone to adipose tissue inflammation that is associated with obesity.
肥胖的特征是慢性低度炎症。在此，我们研究了白色和棕色脂肪细胞之间的内在差异是否会影响巨噬细胞的炎症状态。白色和棕色脂肪细胞通过对 、 、 和 的转录调控及其白细胞介素-6分泌水平来表征。通过在与白色和棕色脂肪细胞进行单培养或间接共培养后，测量白细胞介素-6分泌以及一组炎症基因的转录调控，来评估在静止状态以及用脂多糖/γ干扰素和白细胞介素-4刺激后，经佛波酯分化的U937和THP-1巨噬细胞的炎症特征。与棕色脂肪细胞相比，白色脂肪细胞单培养显示白细胞介素-6分泌增加。与与两种巨噬细胞共培养的棕色脂肪细胞相比，与U937和THP-1巨噬细胞共培养的白色脂肪细胞诱导白细胞介素-6分泌增加得更多。与巨噬细胞共培养的白色脂肪细胞使两种类型的炎症基因表达均增加。相反，与棕色脂肪细胞共培养的巨噬细胞诱导炎症基因表达下调或无变化。脂肪细胞对巨噬细胞的影响似乎与刺激状态无关。棕色脂肪细胞具有抑制巨噬细胞炎症特征的内在能力，而白色脂肪细胞则增强这种炎症特征。这些数据表明，棕色脂肪细胞可能不太容易发生与肥胖相关的脂肪组织炎症。
