Reynolds G E, Griffin J F
Microbiology Department, University of Otago, Dunedin, New Zealand.
Vet Immunol Immunopathol. 1990 Jun;25(2):155-66. doi: 10.1016/0165-2427(90)90032-n.
This study examines the effect of pregnancy on the quantity and isotype of antibody in ewes immunised with a novel primary antigen. The primary and secondary antibody levels to bovine serum albumin (BSA) in alum adjuvant, were compared between non-pregnant ewes and ewes immunised at different stages of pregnancy. Anti-BSA isotype specific responses were measured using an indirect ELISA. Results show the levels of immunoglobulin M (IgM) increased and persisted in response to BSA during pregnancy (P less than 0.05). Secondary immunoglobulin G1 (IgG1) titres were significantly impaired in late pregnancy and during lactation (P less than 0.05). The lower levels of immunoglobulin G2 (IgG2) were unaffected by pregnancy under these experimental conditions. Ewes were also immunised with BSA in alum adjuvant for their primary inoculum and BSA in different adjuvants for the secondary inoculation. Primary IgM persisted at higher levels during pregnancy compared with the response in non-pregnant ewes (P less than 0.05). Following the secondary injection, lower levels of anti-BSA specific IgM, IgG1 and IgG2 antibodies were produced in late pregnancy and during lactation compared with the levels in non-pregnant control ewes (P less than 0.05). Alterations in regulatory T-cell function or effector B-cell activity would most readily explain the qualitative changes in antibody titre observed following primary injection during pregnancy. The results also suggest an associated impairment of immunological memory following primary immunisation during pregnancy.
本研究检测了怀孕对用新型一级抗原免疫的母羊抗体数量和同种型的影响。比较了未怀孕母羊以及在怀孕不同阶段免疫的母羊对明矾佐剂中牛血清白蛋白（BSA）的一级和二级抗体水平。使用间接ELISA检测抗BSA同种型特异性反应。结果显示，怀孕期间对BSA产生反应时，免疫球蛋白M（IgM）水平升高并持续存在（P<0.05）。怀孕后期和哺乳期二级免疫球蛋白G1（IgG1）滴度显著受损（P<0.05）。在这些实验条件下，免疫球蛋白G2（IgG2）的较低水平不受怀孕影响。母羊的初次接种物为明矾佐剂中的BSA，二次接种为不同佐剂中的BSA。与未怀孕母羊的反应相比，怀孕期间一级IgM持续保持较高水平（P<0.05）。二次注射后，与未怀孕对照母羊的水平相比，怀孕后期和哺乳期产生的抗BSA特异性IgM、IgG1和IgG2抗体水平较低（P<0.05）。调节性T细胞功能或效应B细胞活性的改变最容易解释怀孕期间初次注射后观察到的抗体滴度的定性变化。结果还表明，怀孕期间初次免疫后免疫记忆存在相关损伤。
