Hibma M, Griffin J F
Microbiology Dept., University of Otago, Dunedin, New Zealand.
Vet Immunol Immunopathol. 1992 Mar;31(3-4):279-87. doi: 10.1016/0165-2427(92)90015-i.
Experiments were carried out to determine the effects of a range of adjuvants on immunoglobulin M (IgM) and immunoglobulin G (IgG) serum concentrations to a protein antigen administered subcutaneously to farmed female deer following mating. The antibody responses of animals immunised with keyhole limpet hemocyanin (KLH) in Freund's Incomplete Adjuvant (FIA), diethylaminoethyl dextran (DEAE-dextran) and aluminium hydroxide (alum) were compared with the response to antigen administered in the absence of adjuvants. Animals were subsequently challenged with a subcutaneous immunisation of the antigen in saline. Following parturition, the concentration of passively transferred antigen-specific antibody was measured in the serum of the offspring. The polyionic adjuvant, DEAE-dextran, produced the greatest enhancement of both primary and secondary IgG responses to KLH. Offspring suckling from mothers immunised with antigen in DEAE-dextran consequently had higher concentrations of specific antibodies in their serum than other fawns in the experiment. The adjuvants FIA and alum were approximately 20-fold less effective in enhancing antigen-specific IgG than DEAE-dextran but induced greater amounts of antigen-specific IgM. From the results presented in this paper, there is evidence that immunisation of deer during pregnancy may be an effective way of reducing morbidity in both mothers and offspring.
开展了一系列实验，以确定多种佐剂对养殖母鹿交配后皮下注射蛋白质抗原时血清免疫球蛋白M（IgM）和免疫球蛋白G（IgG）浓度的影响。将用弗氏不完全佐剂（FIA）、二乙氨基乙基葡聚糖（DEAE - 葡聚糖）和氢氧化铝（明矾）中的钥孔戚血蓝蛋白（KLH）免疫的动物的抗体反应，与无佐剂情况下注射抗原的反应进行比较。随后，动物接受皮下注射盐水中的抗原的攻击。分娩后，在后代血清中测量被动转移的抗原特异性抗体的浓度。聚离子佐剂DEAE - 葡聚糖对KLH的初次和二次IgG反应增强作用最大。因此，由用DEAE - 葡聚糖中的抗原免疫的母亲哺乳的后代，其血清中的特异性抗体浓度高于实验中的其他小鹿。佐剂FIA和明矾在增强抗原特异性IgG方面的效果比DEAE - 葡聚糖低约20倍，但诱导产生的抗原特异性IgM量更多。根据本文给出的结果，有证据表明孕期给鹿免疫可能是降低母鹿和后代发病率的有效方法。
