Willets Katherine A
Department of Chemistry, University of Texas at Austin, Welch Hall 1.202,105 E 24th ST, A5300, Austin TX 78712 (USA), Fax: (+1) 512-471-0985.
Chemphyschem. 2013 Oct 7;14(14):3186-95. doi: 10.1002/cphc.201300297. Epub 2013 Jun 18.
Surface-enhanced Raman scattering (SERS) is quickly growing as an analytical technique, because it offers both molecular specificity and excellent sensitivity. For select substrates, SERS can even be observed from single molecules, which is the ultimate limit of detection. This review describes recent developments in the field of single-molecule SERS (SM-SERS) with a focus on new tools for characterizing SM-SERS-active substrates and how they interact with single molecules on their surface. In particular, techniques that combine optical spectroscopy and microscopy with electron microscopy are described, including correlated optical and transmission electron microscopy, correlated super-resolution imaging and scanning electron microscopy, and correlated optical microscopy and electron energy loss spectroscopy.
表面增强拉曼散射（SERS）作为一种分析技术正在迅速发展，因为它兼具分子特异性和出色的灵敏度。对于某些特定的底物，甚至可以从单分子中观察到SERS，这是检测的最终极限。本综述描述了单分子SERS（SM-SERS）领域的最新进展，重点关注用于表征具有SM-SERS活性的底物及其与表面单分子相互作用的新工具。特别介绍了将光谱学和显微镜技术与电子显微镜相结合的技术，包括相关光学和透射电子显微镜、相关超分辨率成像和扫描电子显微镜，以及相关光学显微镜和电子能量损失谱。
