Laboratory of Molecular Biology, Fundación Instituto Valenciano de Oncología, Valencia, Spain.
Biomed Res Int. 2013;2013:465179. doi: 10.1155/2013/465179. Epub 2013 May 28.
Prostate cancer (PCa) is a very heterogeneous disease, and there are constraints in its current diagnosis. Serum PSA levels, digital rectal examination (DRE), and histopathologic analysis often drive to overdiagnosis and overtreatment. Since 2005, the presence of the genetic rearrangement between transmembrane-serine protease gene (TMPRSS2) and the erythroblast transformation-specific (ETS) member ERG (v-ets erythroblastosis virus E26 oncogene homolog avian) has been demonstrated in almost half of PCa cases. Both FISH and RT-PCR are useful tools for detecting these rearrangements, but very few comparatives between both techniques have been published. In this study, we included FFPE tumors from 294 PCa patients treated with radical prostatectomy with more than 5 years of followup. We constructed a total of 20 tissue microarrays in order to perform break-apart and tricolor probe FISH approaches that were compared with RT-PCR, showing a concordance of 80.6% (P < 0.001). The presence of TMPRSS2-ERG rearrangement was observed in 56.6% of cases. No association between TMPRSS2-ERG status and clinicopathological parameters nor biochemical progression and clinical progression free survival was found. In conclusion, this study demonstrates that both FISH and RT-PCR are useful tools in the assessment of the TMPRSS2-ERG fusion gene status in PCa patients and that this genetic feature per se lacks prognostic value.
前列腺癌（PCa）是一种非常异质的疾病，目前的诊断存在局限性。血清 PSA 水平、数字直肠检查（DRE）和组织病理学分析常常导致过度诊断和过度治疗。自 2005 年以来，在近一半的 PCa 病例中发现了跨膜丝氨酸蛋白酶基因（TMPRSS2）和红细胞生成特异性（ETS）成员 ERG（v-ets 红细胞生成病毒 E26 癌基因同源物禽）之间的基因重排。荧光原位杂交（FISH）和逆转录聚合酶链反应（RT-PCR）都是检测这些重排的有用工具，但很少有关于这两种技术的比较。在这项研究中，我们纳入了 294 例接受根治性前列腺切除术治疗且随访时间超过 5 年的 PCa 患者的 FFPE 肿瘤。我们总共构建了 20 个组织微阵列，以进行分离和三色探针 FISH 方法，与 RT-PCR 相比，其一致性为 80.6%（P<0.001）。在 56.6%的病例中观察到 TMPRSS2-ERG 重排。TMPRSS2-ERG 状态与临床病理参数、生化进展和临床无进展生存之间没有关联。总之，这项研究表明，FISH 和 RT-PCR 都是评估 PCa 患者 TMPRSS2-ERG 融合基因状态的有用工具，并且这种遗传特征本身缺乏预后价值。
