Belgrave Rodney L, Dickey Meranda M, Arheart Kristopher L, Cray Carolyn
Mid-Atlantic Equine Medical Center, Ringoes, NJ 08551, USA.
J Am Vet Med Assoc. 2013 Jul 1;243(1):113-9. doi: 10.2460/javma.243.1.113.
To compare serum amyloid A (SAA) concentration, plasma fibrinogen concentration, total WBC count, and serum albumin-to-globulin concentration ratio (A:G ratio) in clinically normal (CN) and clinically abnormal (CA) horses.
Prospective cohort study.
111 CN horses and 101 CA horses hospitalized at a specialty clinical practice.
Shortly after admission, a blood sample (20 mL) was collected from each horse for a CBC, serum protein electrophoresis, and determination of plasma fibrinogen concentration; SAA concentration was assessed with a previously validated immunoturbidometric assay. Similar testing of a subset of CA horses was conducted at various points during treatment.
Total WBC count, A:G ratio, and SAA concentration were determined for all 212 horses; data regarding plasma fibrinogen concentration were available for 127 horses (of which 47 were CN and 80 were CA). Median SAA concentration, total WBC count, and plasma fibrinogen concentration and mean A:G ratio differed significantly between CN horses and CA horses. Correlations between these variables were poor to weak. For discrimination of CN horses from CA horses, the SAA assay had sensitivity of 53% and specificity of 94% (diagnostic accuracy, 75%); for the other assessments, accuracy ranged from 59% to 62%. Repeated assessment of SAA concentration in some CA horses revealed a gradual return to normal concentrations.
Results indicated that assessment of SAA concentration can provide valuable information regarding the clinical state of horses and may be more useful for patient monitoring and as a prognostic indicator than are traditional markers of inflammation.
比较临床正常（CN）和临床异常（CA）马匹的血清淀粉样蛋白A（SAA）浓度、血浆纤维蛋白原浓度、白细胞总数以及血清白蛋白与球蛋白浓度比（A:G比）。
前瞻性队列研究。
111匹临床正常马匹和101匹临床异常马匹，均收治于一家专科临床机构。
入院后不久，从每匹马采集20毫升血液样本，用于全血细胞计数、血清蛋白电泳及血浆纤维蛋白原浓度测定；采用先前验证的免疫比浊法评估SAA浓度。在治疗期间的不同时间点，对部分CA马匹进行类似检测。
测定了所有212匹马的白细胞总数、A:G比和SAA浓度；127匹马有血浆纤维蛋白原浓度数据（其中47匹为CN马匹，80匹为CA马匹）。CN马匹和CA马匹之间的SAA浓度中位数、白细胞总数、血浆纤维蛋白原浓度及平均A:G比存在显著差异。这些变量之间的相关性较差至较弱。对于区分CN马匹和CA马匹，SAA检测的敏感性为(53%)，特异性为(94%)（诊断准确率为(75%)）；其他评估的准确率在(59%)至(62%)之间。对部分CA马匹重复检测SAA浓度，结果显示其浓度逐渐恢复正常。
结果表明，评估SAA浓度可为马匹的临床状态提供有价值的信息，与传统炎症标志物相比，在患者监测和预后指标方面可能更有用。
