Kishida E, Oribe M, Mochizuki K, Kojo S, Iguchi H
Department of Life and Health Sciences, Hyogo University of Teacher Education, Japan.
Biochim Biophys Acta. 1990 Jul 16;1045(2):187-8. doi: 10.1016/0005-2760(90)90149-r.
A new HPLC method to determine malondialdehyde (MDA) was developed. Malondialdehyde was reacted with alpha-N-benzoyl-L-arginine ethyl ester and converted into alpha-N-benzoyl-delta-N-(2-pyrimidinyl)-L-ornithine ethyl ester, which is sufficiently hydrophobic to allow us its specific determination utilizing a reversed-phase C18 column at the level of 1 pmol.
