高效液相色谱法测定植物油中的丙二醛
High performance liquid chromatographic determination of malondialdehyde in vegetable oils.
作者信息
Hirayama T, Yamada N, Nohara M, Fukui S
出版信息
J Assoc Off Anal Chem. 1983 Mar;66(2):304-8.
PMID:6860411
Abstract
A simple high performance liquid chromatographic (HPLC) method has been developed for determining malondialdehyde (MDA) in vegetable oils. MDA was reacted with dansyl hydrazine in an acidic medium, and the product, 1-dansyl-pyrazole, was determined by HPLC, using a Zorbax sil column with mixed mobile phase of n-hexane-methylene chloride. MDA can be determined as 1-dansyl-pyrazole by fluorometric detection at a level of 0.01 ppm in vegetable oils.
摘要
已开发出一种简单的高效液相色谱（HPLC）方法用于测定植物油中的丙二醛（MDA）。丙二醛在酸性介质中与丹磺酰肼反应，产物1-丹磺酰基吡唑用HPLC测定，采用Zorbax硅胶柱，以正己烷 - 二氯甲烷的混合流动相。通过荧光检测，植物油中丙二醛可作为1-丹磺酰基吡唑以0.01 ppm的水平测定。
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