Yu L W, Latriano L, Duncan S, Hartwick R A, Witz G
Anal Biochem. 1986 Aug 1;156(2):326-33. doi: 10.1016/0003-2697(86)90261-7.
A reversed-phase HPLC method is described for the separation and analysis of the thiobarbituric acid (TBA) adducts of the reactive aldehydes muconaldehyde (MUC) and malonaldehyde (MDA). The TBA adduct of malonaldehyde was synthesized, purified, and its structure elucidated, for use as standard in quantitative HPLC studies. A detection limit of 1 X 10(-14) mol was achieved for the MUC:TBA and MDA:TBA adducts using the double monochromator fluorometric detector, 7 X 10(-13) mol was the detection limit using a variable wavelength uv-visible detector. Direct on-line identification of the eluting aldehyde:TBA adducts was achieved by the use of a diode-array uv-visible detector. The chromatographic behavior of the adducts under different mobile phase conditions was also examined. This HPLC methodology was used for the identification of muconaldehyde as a product of benzene oxidation in a hydroxyl radical generating system.
本文描述了一种反相高效液相色谱法，用于分离和分析活性醛粘康醛（MUC）和丙二醛（MDA）的硫代巴比妥酸（TBA）加合物。合成、纯化了丙二醛的TBA加合物，并阐明了其结构，用作高效液相色谱定量研究的标准品。使用双单色荧光检测器时，MUC:TBA和MDA:TBA加合物的检测限为1×10⁻¹⁴ mol，使用可变波长紫外可见检测器时，检测限为7×10⁻¹³ mol。通过使用二极管阵列紫外可见检测器，可直接在线鉴定洗脱的醛:TBA加合物。还研究了加合物在不同流动相条件下的色谱行为。该高效液相色谱方法用于鉴定粘康醛是羟基自由基产生系统中苯氧化的产物。
