Department of Horticulture & Food Science, Faculty of Agriculture and Horticulture, University of Craiova, A.I. Cuza 13, 200585 Craiova, Romania.
Food Chem. 2013 Nov 15;141(2):961-6. doi: 10.1016/j.foodchem.2013.03.105. Epub 2013 Apr 13.
Ethanolic extracts prepared from the fruits of three cultivars of black currant ('Record', 'Blackdown' and 'Ronix') macerated in three concentrations (40%, 60% and 96%) of aqueous ethanol were investigated for their anthocyanins profile, total phenolics and antioxidant activity. Nine individual anthocyanins were detected and quantified by using HPLC-MS in ethanolic extracts of black currants, i.e. delphinidin 3-glucoside, delphinidin 3-rutinoside, cyanidin 3-glucoside, cyanidin 3-rutinoside, petunidin 3-rutinoside, pelargonidin 3-rutinoside, peonidin 3-rutinoside, petunidin 3-(6-coumaroyl)-glucoside and cyanidin 3-(6-coumaroyl)-glucoside. The antioxidant activity of the alcoholic extracts was investigated by the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method while the total phenolic content was determined by using the Folin-Ciocalteu assay. The highest anthocyanins content was found in the extracts made in 60% ethanol while total phenolics content was highest at 96% ethanol concentration. The extraction yield of individual anthocyanins was differently influenced by the rise of the ethanolic concentration. Maximum extraction yield of most individual anthocyanins was reached at 60% ethanol concentration except the 3-(6-coumaroyl)-glucoside of cyanidin and petunidin whose maximum extraction occurred at 96% ethanol concentration.
从浸渍在 3 种浓度（40%、60%和 96%）乙醇水溶液中的 3 种黑加仑品种（“Record”、“Blackdown”和“Ronix”）果实中制备的醇提物，用于研究其花色苷谱、总酚和抗氧化活性。通过 HPLC-MS 在黑加仑的醇提物中检测和定量了 9 种单体花色苷，即矢车菊素 3-葡萄糖苷、矢车菊素 3-鼠李糖苷、飞燕草素 3-葡萄糖苷、飞燕草素 3-鼠李糖苷、锦葵素 3-鼠李糖苷、天竺葵素 3-鼠李糖苷、矢车菊素 3-（6-咖啡酰基）葡萄糖苷和飞燕草素 3-（6-咖啡酰基）葡萄糖苷。通过 2,2'-二苯基-1-苦基肼（DPPH）自由基清除法研究了醇提取物的抗氧化活性，而总酚含量则通过 Folin-Ciocalteu 测定法确定。在 60%乙醇中提取的花色苷含量最高，而在 96%乙醇浓度下总酚含量最高。单体花色苷的提取率随乙醇浓度的升高而不同。除了矢车菊素和锦葵素的 3-（6-咖啡酰基）葡萄糖苷在 96%乙醇浓度下达到最大提取量外，大多数单体花色苷的最大提取量均在 60%乙醇浓度下达到。
