Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU), Paseo de la Universidad, 7, 01006 Vitoria, Spain.
Food Chem. 2013 Nov 15;141(2):1530-5. doi: 10.1016/j.foodchem.2013.03.085. Epub 2013 Apr 13.
The effect of resveratrol on thermogenesis in skeletal muscle and interscapular brown adipose tissue (IBAT) was investigated. Rats were fed an obesogenic diet supplemented with resveratrol (30mg/kg/day) or not supplemented for 6weeks. Resveratrol intake led to increased gene expression of mitochondrial-transcription-factor-A (TFAM), mitochondrial-protein-cytochrome-C-oxidase subunit-2 (COX2), sirtuin-1 (SIRT1), peroxisome-proliferator-activated-receptor-β/δ (PPARβ/δ) and proliferator-activated-receptor-gamma-coactivator1-α (PGC-1α) in IBAT and increased UCP1protein expression; however, peroxisome-proliferator-activated-receptor-α (PPARα) expression remained unchanged. In gastrocnemius muscle, resveratrol increased the gene expression of TFAM and COX2; however, no changes were observed in levels of SIRT1, PGC-1α and PPARβ/δ. Acetylated-PGC-1α was decreased in the resveratrol-treated group, indicating a higher level of activation, and a significant increase of UCP3 protein expression was observed in this group. The increases in UCP protein expression in two important thermogenic tissues after resveratrol treatment may contribute to increased whole-body energy dissipation, which may help to better understand the body-fat lowering effect of this polyphenol.
研究了白藜芦醇对骨骼肌和肩胛间棕色脂肪组织（IBAT）产热的影响。大鼠喂食添加白藜芦醇（30mg/kg/天）或不添加的肥胖症饮食 6 周。白藜芦醇的摄入导致 IBAT 中线粒体转录因子 A（TFAM）、线粒体蛋白细胞色素 C 氧化酶亚基 2（COX2）、沉默调节蛋白 1（SIRT1）、过氧化物酶体增殖物激活受体-β/δ（PPARβ/δ）和过氧化物酶体增殖物激活受体-γ共激活因子 1-α（PGC-1α）的基因表达增加，并增加 UCP1 蛋白表达；然而，过氧化物酶体增殖物激活受体-α（PPARα）的表达保持不变。在比目鱼肌中，白藜芦醇增加了 TFAM 和 COX2 的基因表达；然而，SIRT1、PGC-1α 和 PPARβ/δ 的水平没有变化。乙酰化 PGC-1α 在白藜芦醇处理组中减少，表明激活水平更高，并且在该组中观察到 UCP3 蛋白表达显著增加。白藜芦醇处理后两种重要产热组织中 UCP 蛋白表达的增加可能有助于增加全身能量消耗，这有助于更好地理解这种多酚降低体脂肪的作用。
