Vásquez-Reyes Sarai, Bernal-Gámez Miranda, Domínguez-Chávez Jorge, Mondragón-Vásquez Karina, Sánchez-Tapia Mónica, Ordaz Guillermo, Granados-Portillo Omar, Coutiño-Hernández Diana, Barrera-Gómez Paulina, Torres Nimbe, Tovar Armando R
Departamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, CDMX, México 14080, Mexico.
Facultad de Bioanálisis Región Veracruz, Universidad Veracruzana, Agustín de Iturbide, Veracruz 91700, Mexico.
Nutrients. 2024 Dec 23;16(24):4425. doi: 10.3390/nu16244425.
BACKGROUND/OBJECTIVE: In recent studies, it has been shown that dietary bioactive compounds can produce health benefits; however, it is not known whether an improvement in solubility can enhance their biological effects. Thus, the aim of this work was to study whether co-amorphous (CoA) naringenin or fisetin with enhanced solubility modify glucose and lipid metabolism, thermogenic capacity and gut microbiota in mice fed a high-fat, high-sucrose (HFSD) diet.
Mice were fed with an HFSD with or without CoA-naringenin or CoA-fisetin for 3 months. Body weight, food intake, body composition, glucose tolerance, hepatic lipid composition and gut microbiota were assessed.
CoA-naringenin demonstrated significant reductions in fat-mass gain, improved cholesterol metabolism, and enhanced glucose tolerance. Mice treated with CoA-naringenin gained 45% less fat mass and exhibited improved hepatic lipid profiles, with significant reductions seen in liver triglycerides and cholesterol. Additionally, both CoA-flavonoids increased oxygen consumption (VO), contributing to enhanced energy expenditure and improved metabolic flexibility. Thermogenic activation, indicated by increased UCP1 and PGC-1α levels, was observed with CoA-fisetin, supporting its role in fat oxidation and adipocyte size reduction. Further, both CoA-flavonoids modulated gut microbiota, restoring diversity and promoting beneficial bacteria, such as , which has been linked to improved metabolic health.
These findings suggest that co-amorphous naringenin or fisetin offers promising applications in improving solubility, metabolic health, and thermogenesis, highlighting the potential of both as therapeutic agents against obesity and related disorders.
背景/目的：在最近的研究中，已表明膳食生物活性化合物可产生健康益处；然而，尚不清楚溶解度的提高是否能增强其生物学效应。因此，本研究的目的是探究具有更高溶解度的共无定形（CoA）柚皮苷或非瑟酮是否会改变高脂高糖（HFSD）饮食喂养小鼠的葡萄糖和脂质代谢、产热能力及肠道微生物群。
给小鼠喂食含或不含CoA-柚皮苷或CoA-非瑟酮的HFSD饮食3个月。评估体重、食物摄入量、身体组成、葡萄糖耐量、肝脏脂质组成和肠道微生物群。
CoA-柚皮苷显著降低了脂肪量增加，改善了胆固醇代谢，并增强了葡萄糖耐量。用CoA-柚皮苷处理的小鼠脂肪量增加减少了45%，肝脏脂质谱得到改善，肝脏甘油三酯和胆固醇显著降低。此外，两种CoA-类黄酮均增加了耗氧量（VO），有助于增加能量消耗和改善代谢灵活性。观察到CoA-非瑟酮可激活产热，表现为UCP1和PGC-1α水平升高，这支持了其在脂肪氧化和减少脂肪细胞大小方面的作用。此外，两种CoA-类黄酮均调节肠道微生物群，恢复其多样性并促进有益细菌生长，如 ，这与改善代谢健康有关。
这些发现表明，共无定形柚皮苷或非瑟酮在改善溶解度、代谢健康和产热方面具有广阔的应用前景，突出了二者作为抗肥胖及相关疾病治疗药物的潜力。
