Department of Chemical Engineering, Virginia Tech, Blacksburg, VA 24061, USA.
Acta Biomater. 2013 Sep;9(9):8037-45. doi: 10.1016/j.actbio.2013.06.014. Epub 2013 Jun 19.
Calcium phosphate ceramics (CPCs) have been widely used as biomaterials for the regeneration of bone tissue because of their ability to induce osteoblastic differentiation in progenitor cells. Despite the progress made towards fabricating CPCs possessing a range of surface features and chemistries, the influence of material properties in orchestrating cellular events such as adhesion and differentiation is still poorly understood. Specifically, questions such as why certain CPCs may be more osteoinductive than others, and how material properties contribute to osteoinductivity/osteoconductivity remain unanswered. Therefore, this review article systematically discusses the effects of the physical (e.g. surface roughness) and chemical properties (e.g. solubility) of CPCs on protein adsorption, cell adhesion and osteoblastic differentiation in vitro. The review also provides a summary of possible signaling pathways involved in osteoblastic differentiation in the presence of CPCs. In summary, these insights on the contribution of material properties towards osteoinductivity and the role of signaling molecules involved in osteoblastic differentiation can potentially aid the design of CPC-based biomaterials that support bone regeneration without the need for additional biochemical supplements.
磷酸钙陶瓷（CPCs）因其能够诱导祖细胞成骨细胞分化，已被广泛用作骨组织再生的生物材料。尽管在制造具有各种表面特征和化学性质的 CPCs 方面取得了进展，但材料性质在调节细胞事件（如黏附与分化）方面的影响仍知之甚少。具体而言，某些 CPCs 为何比其他 CPCs 更具成骨性，以及材料性质如何促成成骨性/骨传导性等问题仍未得到解答。因此，本文系统地讨论了 CPCs 的物理（例如表面粗糙度）和化学性质（例如溶解度）对蛋白质吸附、细胞黏附和体外成骨细胞分化的影响。本文还总结了 CPC 存在时涉及成骨细胞分化的可能信号通路。总之，这些关于材料性质对成骨性的贡献以及参与成骨细胞分化的信号分子的作用的见解，可能有助于设计基于 CPC 的生物材料，在无需额外生化补充的情况下支持骨再生。
