Department of Agronomy, Iowa State University, Ames, IA, USA.
Theor Appl Genet. 2013 Sep;126(9):2391-403. doi: 10.1007/s00122-013-2143-4. Epub 2013 Jun 23.
Sudden death syndrome (SDS) is an important soybean [Glycine max (L) Merrill] disease caused by the soilborne fungus Fusarium virguliforme. Currently, 14 quantitative trait loci (QTL) had been confirmed associated with resistance or tolerance to SDS. The objective of the study was to evaluate usefulness of 10 of these QTL in controlling disease expression. Six populations were developed providing a total of 321 F2-derived lines for the study. Recombinant inbred lines (RIL) used as parents were obtained from populations of 'Essex' × 'Forrest' (EF), 'Flyer' × 'Hartwig' (FH), and 'Pyramid' × 'Douglas' (PD). Disease resistance was evaluated in the greenhouse at three different planting times, each with four replications, using sorghum infested with F. virguliforme homogeneously mixed in the soil (Luckew et al., Crop Sci 52:2215-2223, 2012). Four disease assessment criteria-foliar disease incidence (DI), foliar leaf scorch disease severity (DS), area under the disease progress curve (AUDPC), and root rot severity-were used. QTL were identified in more than one of the disease assessment criteria, mainly associated with lines in the most resistant categories. Five QTL (qRfs4, qRfs5, qRfs7, qRfs12, and Rfs16) were associated with at least one of the disease assessments across multiple populations. Of the five, qRfs4 was associated with DI, AUDPC, and root rot severity, and Rfs16 with AUDPC and root rot severity. The findings suggest it may be possible for plant breeders to focus on stacking a subset of the previously identified QTL to improve resistance to SDS in soybean.
突发性死亡综合征（SDS）是一种由土壤传播真菌尖孢镰刀菌引起的重要大豆[ Glycine max （L）Merrill]疾病。目前，已经确定了与 SDS 抗性或耐受性相关的 14 个数量性状位点（QTL）。本研究的目的是评估其中 10 个 QTL 在控制疾病表达方面的有用性。共开发了 6 个群体，共提供了 321 条 F2 衍生系进行研究。作为亲本使用的重组自交系（RIL）是从 'Essex'×'Forrest'（EF）、'Flyer'×'Hartwig'（FH）和 'Pyramid'×'Douglas'（PD）的群体中获得的。在温室中，使用均匀混合在土壤中的感染尖孢镰刀菌的高粱（Luckew 等人，作物科学 52:2215-2223,2012），在三个不同的种植时间进行了 4 次重复的抗病性评估。使用了 4 种疾病评估标准-叶片疾病发生率（DI）、叶片叶烧疾病严重度（DS）、疾病进展曲线下的面积（AUDPC）和根腐严重度。在多个疾病评估标准中鉴定到了 5 个 QTL（qRfs4、qRfs5、qRfs7、qRfs12 和 Rfs16），主要与最具抗性类别的系相关。在五个 QTL 中，qRfs4 与 DI、AUDPC 和根腐严重度有关，Rfs16 与 AUDPC 和根腐严重度有关。研究结果表明，植物育种家可能有可能集中于叠加先前鉴定的 QTL 的子集，以提高大豆对 SDS 的抗性。
