Institute of Basic Medical Sciences, PLA General Hospital, the PLA Medical College, Beijing, 100853, China.
Front Med. 2013 Sep;7(3):345-53. doi: 10.1007/s11684-013-0282-2. Epub 2013 Jun 23.
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) possess various advantageous properties, including self-renewal, extended proliferation potential, multi-lineage differentiation potential and capacity for differentiating into sweat gland-like cells in certain conditions. However, little is known about the effect of clinical-grade culture conditions on these properties and on the differentiative potential of hUC-MSCs. In this study, we sought to investigate the properties of hUC-MSCs expanded with animal serum free culture media (ASFCM) in order to determine their potential for differentiation into sweat gland-like cells. We found that primary cultures of hUC-MSCs could be established with ASFCM. Moreover, cells cultured in ASFCM showed vigorous proliferation comparable to those of cells grown in classical culture conditions containing fetal bovine serum (FBS). Morphology of hUC-MSCs cultured in ASFCM was comparable to those of cells grown under classical culture conditions, and hUC-MSCs grown in both of the two culture conditions tested showed the typical antigen profile of MSCs-positive for CD29, CD44, CD90, and CD105, and negative for CD34 and CD45, as expected. Chromosomal aberration assay revealed that the cells were stable after long-term culture under both culture conditions. Like normal cultured MSCs, hUC-MSCs induced under ASFCM conditions exhibited expression of the same markers (CEA, CK14 and CK19) and developmental genes (EDA and EDAR) that are characteristic of normal sweat gland cells. Taken together, our findings indicate that the classical culture medium used to differentiate hUC-MSCs into sweat gland-like cells can be replaced safely by ASFCM for clinical purposes.
人脐带间充质干细胞（hUC-MSCs）具有多种优势特性，包括自我更新、延长增殖潜能、多向分化潜能，并能在特定条件下分化为汗腺样细胞。然而，对于临床级培养条件对这些特性和 hUC-MSCs 的分化潜能的影响，人们知之甚少。在这项研究中，我们试图研究使用无动物血清培养培养基（ASFCM）扩增的 hUC-MSCs 的特性，以确定其分化为汗腺样细胞的潜力。我们发现，hUC-MSCs 可以使用 ASFCM 建立原代培养。此外，在 ASFCM 中培养的细胞表现出与在含有胎牛血清（FBS）的经典培养条件下生长的细胞相当的旺盛增殖能力。在 ASFCM 中培养的 hUC-MSCs 的形态与在经典培养条件下生长的细胞相似，在这两种培养条件下生长的 hUC-MSCs 均表现出 MSC 典型的抗原表型，即 CD29、CD44、CD90 和 CD105 阳性，CD34 和 CD45 阴性，这与预期相符。染色体畸变试验表明，在这两种培养条件下长期培养后，细胞均保持稳定。与正常培养的 MSC 一样，在 ASFCM 条件下诱导的 hUC-MSCs 表达了与正常汗腺细胞特征性相关的相同标记物（CEA、CK14 和 CK19）和发育基因（EDA 和 EDAR）。综上所述，我们的研究结果表明，可安全地用 ASFCM 替代经典培养基，将 hUC-MSCs 分化为汗腺样细胞用于临床目的。
