Department of Dermatology, Seoul National University College of Medicine, 28 Yongon-dong, Seoul, 110-744, Korea; Acne Research Laboratory, Seoul National University Hospital, Seoul, Korea.
Br J Dermatol. 2013 Nov;169(5):1152-5. doi: 10.1111/bjd.12486.
The precise roles of Propionibacterium acnes and other anaerobic bacteria in the pathogenesis of acne are still unclear. Recent studies have shown that P. acnes can be further classified into several phylotypes with distinct phenotypes and virulence. Their distribution patterns in acne lesions have rarely been demonstrated.
To analyse distribution patterns of P. acnes phylotypes and Peptostreptococcus species on the skin surface of patients with acne and healthy controls, and in comedones, papules and pustules from patients.
A total of 370 samples from 95 patients with acne and 65 samples from 65 healthy controls were investigated. Three P. acnes phylotypes and three Peptostreptococcus species were identified by polymerase chain reaction primarily using type-specific primers.
There was no significant difference in the microflora of the skin surface samples between patients with acne and healthy controls. In acne lesions, distribution patterns between skin surface and comedonal lesions were similar, but they were significantly different from those of both papules and pustules. In the inflammatory acne lesions, the proportion of type IA P. acnes was increased, while those of type IB and II were decreased. The proportion of Peptostreptococcus species was also increased significantly in the inflammatory lesions.
At the precision of this qualitative study, our results do not provide any evidence that different phylotypes in the surface microflora might be important in triggering acne. However, type IA P. acnes and Peptostreptococcus species might be more closely associated with inflammatory acne lesions.
痤疮丙酸杆菌和其他厌氧菌在痤疮发病机制中的确切作用仍不清楚。最近的研究表明，痤疮丙酸杆菌可以进一步分为具有不同表型和毒力的几个菌型。它们在痤疮病变中的分布模式很少被证明。
分析痤疮患者和健康对照者皮肤表面、粉刺、丘疹和脓疱中痤疮丙酸杆菌菌型和消化链球菌属种的分布模式。
共检测了 95 例痤疮患者的 370 个样本和 65 例健康对照者的 65 个样本。采用聚合酶链反应（PCR），主要使用特定于类型的引物，对三种痤疮丙酸杆菌菌型和三种消化链球菌属种进行鉴定。
痤疮患者和健康对照者皮肤表面样本的微生物群无显著差异。在痤疮病变中，皮肤表面和粉刺病变之间的分布模式相似，但与丘疹和脓疱病变显著不同。在炎症性痤疮病变中，IA 型痤疮丙酸杆菌的比例增加，而 IB 和 II 型的比例减少。炎症性病变中消化链球菌属种的比例也显著增加。
在本定性研究的精度范围内，我们的结果没有提供任何证据表明表面微生物群中的不同菌型可能在引发痤疮方面很重要。然而，IA 型痤疮丙酸杆菌和消化链球菌属种可能与炎症性痤疮病变更密切相关。
