Department of Medical Biosciences/Pathology, Umeå University, Umeå S-90185, Sweden.
Br J Dermatol. 2012 Jul;167(1):50-8. doi: 10.1111/j.1365-2133.2012.10897.x. Epub 2012 Jun 1.
Acne vulgaris is a disorder of the sebaceous follicles. Propionibacterium acnes can be involved in inflammatory acne.
This case-control study aimed at investigating the occurrence and localization of P. acnes in facial biopsies in acne and to characterize the P. acnes phylotype in skin compartments.
Specific monoclonal and polyclonal antibodies were applied to skin biopsies of 38 patients with acne and matching controls to localize and characterize P. acnes and to determine expression of co-haemolysin CAMP factor, a putative virulence determinant.
Follicular P. acnes was demonstrated in 18 (47%) samples from patients with acne and eight (21%) control samples [odds ratio (OR) 3·37, 95% confidence interval (CI) 1·23-9·23; P = 0·017]. In 14 (37%) samples from patients with acne, P. acnes was visualized in large macrocolonies/biofilms in sebaceous follicles compared with only five (13%) control samples (OR 3·85, 95% CI 1·22-12·14; P = 0·021). Macrocolonies/biofilms consisting of mixed P. acnes phylotypes expressing CAMP1 were detected in both case and control samples. Only four samples tested positive for the presence of Staphylococcus spp. and fungi were not observed.
We have for the first time visualized different P. acnes phylotypes in macrocolonies/biofilms in sebaceous follicles of skin biopsies. Our results support the hypothesis that P. acnes can play a role in the pathogenesis of acne as acne samples showed a higher prevalence of follicular P. acnes colonization, both in terms of follicles containing P. acnes and the greater numbers of bacteria in macrocolonies/biofilms than in control samples.
寻常痤疮是一种皮脂腺滤泡的疾病。痤疮丙酸杆菌可能参与了炎症性痤疮的发生。
本病例对照研究旨在调查寻常痤疮面部活检中痤疮丙酸杆菌的发生和定位，并对皮肤各部位的痤疮丙酸杆菌的菌株进行特征描述。
应用特异性单克隆和多克隆抗体对 38 例寻常痤疮患者和匹配对照的皮肤活检进行检测，以定位和鉴定痤疮丙酸杆菌，并确定可能的毒力决定因素细胞溶素 CAMP 因子的表达情况。
在 18 例（47%）痤疮患者的样本中检测到毛囊内的痤疮丙酸杆菌，而在 8 例（21%）对照样本中则检测到[比值比（OR）3.37，95%置信区间（CI）1.23-9.23；P=0.017]。在 14 例（37%）痤疮患者的样本中，与仅 5 例（13%）对照样本相比，在皮脂腺滤泡中观察到大量的痤疮丙酸杆菌的大聚集体/生物膜（OR 3.85，95%CI 1.22-12.14；P=0.021）。在病例和对照样本中均检测到表达 CAMP1 的混合痤疮丙酸杆菌菌株的大聚集体/生物膜。只有 4 个样本检测到金黄色葡萄球菌阳性，而未观察到真菌。
我们首次在皮肤活检的皮脂腺滤泡中观察到不同的痤疮丙酸杆菌的菌株形成大聚集体/生物膜。我们的研究结果支持这样一种假说，即痤疮丙酸杆菌可能在寻常痤疮的发病机制中起作用，因为痤疮样本显示出更高的毛囊内痤疮丙酸杆菌定植率，无论是在含有痤疮丙酸杆菌的毛囊数量方面，还是在大聚集体/生物膜中的细菌数量方面，都比对照样本高。
