Laboratory of Plant Biochemistry and Genetics, Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz, ESALQ, USP, Piracicaba, SP 13400-970, Brazil.
J Proteomics. 2013 Nov 20;93:107-16. doi: 10.1016/j.jprot.2013.05.039. Epub 2013 Jun 22.
This work evaluates the activity of a few key enzymes involved in combating reactive oxygen species (ROS), such as ascorbate peroxidase (EC 1.11.1.11), catalase (EC 1.11.1.6), glutathione reductase (EC 1.6.4.2), and superoxide dismutase (EC 1.15.1.1), as well as the concentration of malondialdehyde and hydrogen peroxide in transgenic and non-transgenic soybean leaves. Additionally, differential protein species from leaves of both genotypes were evaluated by applying a regulation factor of ≥1.8 to further corroborate the hypothesis that genetic modification itself can be a stress factor for these plants. For this task, transgenic soybean plants were obtained from seeds modified with the cp4EPSPS gene. The results revealed higher activities of all evaluated enzymes in transgenic than in non-transgenic soybean leaves (ranging from 13.8 to 70.1%), as well as higher concentrations of malondialdehyde and hydrogen peroxide in transgenic soybean leaves, clearly indicating a condition of oxidative stress established in the transgenic genotype. Additionally, 47 proteins were differentially abundant when comparing the leaves of both plants, with 26 species accurately identified, including the protein involved in the genetic modification (CP4EPSPS). From these results, it is possible to conclude that the plant is searching for a new equilibrium to maintain its metabolism because the stress condition is being maintained within levels that can be tolerated by the plant.
The present paper is the first one in the literature where are shown translational aspects involving plant stress and the genetic modification for soybean involving the cp4 EPSPS gene. The main biological importance of this work is to make possible the demystification of the genetic modification, allowing answers for some questions that still remain unknown, and enlarge our knowledge about genetically modified organisms. This article is part of a Special Issue entitled: Translational Plant Proteomics.
本研究评估了几种参与清除活性氧（ROS）的关键酶的活性，如抗坏血酸过氧化物酶（EC 1.11.1.11）、过氧化氢酶（EC 1.11.1.6）、谷胱甘肽还原酶（EC 1.6.4.2）和超氧化物歧化酶（EC 1.15.1.1），以及转基因和非转基因大豆叶片中的丙二醛和过氧化氢浓度。此外，还通过应用≥1.8 的调节因子评估了两种基因型叶片中的差异蛋白种类，以进一步证实遗传修饰本身可能是这些植物的应激因素这一假设。为此任务，获得了用 cp4EPSPS 基因修饰的种子的转基因大豆植物。结果表明，与非转基因大豆叶片相比，所有评估酶在转基因大豆叶片中的活性更高（范围为 13.8 至 70.1%），并且转基因大豆叶片中的丙二醛和过氧化氢浓度更高，这清楚地表明了转基因基因型中建立的氧化应激状态。此外，在比较两种植物的叶片时，有 47 种蛋白质的丰度存在差异，其中 26 种物种被准确鉴定，包括参与遗传修饰的蛋白质（CP4EPSPS）。从这些结果可以得出结论，植物正在寻找新的平衡来维持其新陈代谢，因为植物可以承受的水平内维持着应激状态。
本文是文献中首次展示涉及大豆 cp4 EPSPS 基因遗传修饰的植物应激和翻译方面的内容。这项工作的主要生物学意义在于使遗传修饰能够得到解释，为一些仍然未知的问题提供答案，并扩大我们对转基因生物的认识。本文是特刊题为“翻译植物蛋白质组学”的一部分。
