骨骼肌线粒体对L-乳酸的氧化作用。

L-lactate oxidation by skeletal muscle mitochondria.

Szczesna-Kaczmarek A

Department of Bioenergetics, Jedrzej Sniadecki, Academy of Physical Education, Gdańsk-Oliwa, Poland.

Int J Biochem. 1990;22(6):617-20. doi: 10.1016/0020-711x(90)90038-5.

Abstract

Mitochondria isolated from rat skeletal muscle possess lactate dehydrogenase which is involved in direct oxidation of L-lactate in the presence of external NAD. 2. L-lactate oxidation can be stimulated in a reversible manner by ADP. 3. Mitochondrial lactate oxidation is sensitive to oxamate-inhibitor of LDH, alpha-cyano-3-hydroxy-cinnamate-pyruvate translocase inhibitor and respiratory chain inhibitors (rotenone, antimycin A, KCN). 4. In the same conditions the mitochondria did not oxidize pyruvate in the absence of malate, whereas, oxidize pyruvate plus external NADH in an uncoupling manner.

摘要

从大鼠骨骼肌分离出的线粒体含有乳酸脱氢酶，该酶在存在外部烟酰胺腺嘌呤二核苷酸（NAD）的情况下参与L-乳酸的直接氧化。2. 二磷酸腺苷（ADP）可以以可逆的方式刺激L-乳酸的氧化。3. 线粒体乳酸氧化对乳酸脱氢酶的草氨酸抑制剂、α-氰基-3-羟基肉桂酸 - 丙酮酸转运酶抑制剂和呼吸链抑制剂（鱼藤酮、抗霉素A、氰化钾）敏感。4. 在相同条件下，线粒体在没有苹果酸的情况下不氧化丙酮酸，而以解偶联的方式氧化丙酮酸加外部还原型烟酰胺腺嘌呤二核苷酸（NADH）。