Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Nat Neurosci. 2013 Jul;16(7):798-804. doi: 10.1038/nn.3418. Epub 2013 Jun 25.
The complexity of the central nervous system calls for immunocytochemical procedures that allow target proteins to be localized with high precision and with opportunities for quantitation. Immunogold procedures stand out as particularly powerful in this regard. Although these procedures have found wide application in the neuroscience community, they present limitations and pitfalls that must be taken into account. At the same time, these procedures offer potentials that remain to be fully realized.
中枢神经系统的复杂性要求免疫细胞化学技术能够高精度定位目标蛋白,并提供定量机会。免疫金技术在这方面表现尤为突出。尽管这些技术在神经科学界得到了广泛应用,但它们也存在一些必须考虑的局限性和陷阱。同时,这些技术也具有尚未充分实现的潜力。