Tomasi Cristiano, Tessarolo Francesco, Caola Iole, Wennström Jan, Nollo Giandomenico, Berglundh Tord
Department of Periodontology, Institute of Odontology, The Sahlgrenska Academy at University of Gothenburg, Göteborg, Sweden.
Clin Oral Implants Res. 2014 Sep;25(9):997-1003. doi: 10.1111/clr.12223. Epub 2013 Jun 26.
To apply a novel human model to evaluate the morphogenesis of the mucosal attachment to implants.
Twenty one patients receiving implant-supported single-tooth replacement were enrolled in this study. After implant installation, a custom-designed experimental abutment was connected to the implant. Soft tissue biopsies representing 2, 4, 8 or 12 weeks of healing were collected by the use of a circular cutting device and prepared for histological analysis.
The soft tissue biopsies were retrieved, preserved and processed with a technique that was safe and reproducible. The results from the histological analysis in regards to dimensional and qualitative changes in the mucosa over time were consistent with those reported from animal experiments. At 8 weeks, the soft tissue dimension was about 3.6 mm and included a barrier epithelium of 1.9 mm and a connective tissue portion of 1.7 mm. Similar dimensions were found at 12 weeks.
It is suggested that the new human model provides advantages in terms of cost-effectiveness in research as well as from ethical aspects and should be considered as an alternative to pre-clinical in vivo studies in animals.
应用一种新型人体模型评估种植体黏膜附着的形态发生。
本研究纳入21例接受种植体支持单颗牙修复的患者。种植体植入后，将定制设计的实验基台连接到种植体上。使用圆形切割装置收集代表愈合2、4、8或12周的软组织活检样本，并准备进行组织学分析。
软组织活检样本得以安全且可重复的技术获取、保存和处理。组织学分析关于黏膜随时间的尺寸和质量变化的结果与动物实验报告的结果一致。在8周时，软组织尺寸约为3.6毫米，包括1.9毫米的屏障上皮和1.7毫米的结缔组织部分。在12周时发现了类似的尺寸。
建议这种新的人体模型在研究的成本效益以及伦理方面具有优势，应被视为动物临床前体内研究的替代方法。
