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Genetic analysis and development of species-specific PCR assays based on ITS-1 region of rRNA in bovine Eimeria parasites.基于牛艾美耳球虫 rRNA ITS-1 区的遗传分析和种特异性 PCR 检测方法的建立。
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Phylogenetic relationships among Eimeria spp. (Apicomplexa, Eimeriidae) infecting rabbits: evolutionary significance of biological and morphological features.感染家兔的艾美耳球虫（顶复门，艾美耳科）之间的系统发育关系：生物学和形态学特征的进化意义
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A novel procedure for total nucleic acid extraction from small numbers of Eimeria species oocysts.一种从少量艾美耳属球虫卵囊中提取总核酸的新方法。
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Clustal W and Clustal X version 2.0.Clustal W和Clustal X 2.0版本
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Phylogenetic relationships among rodent Eimeria species determined by plastid ORF470 and nuclear 18S rDNA sequences.通过质体ORF470和核18S rDNA序列确定的啮齿动物艾美耳球虫物种之间的系统发育关系。
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Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences.利用完整的小亚基核糖体DNA序列推断感染家鸡的8种艾美耳球虫之间的系统发育关系。
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基于核18S rDNA序列确定寄生于牛的艾美耳球虫种类之间的系统发育关系。

Determination of phylogenetic relationships among Eimeria species, which parasitize cattle, on the basis of nuclear 18S rDNA sequence.

作者信息

Kokuzawa Takuya, Ichikawa-Seki Madoka, Itagaki Tadashi

机构信息

Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.

出版信息

J Vet Med Sci. 2013 Nov;75(11):1427-31. doi: 10.1292/jvms.13-0273. Epub 2013 Jun 26.

DOI:10.1292/jvms.13-0273
PMID:23803494
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3942970/
Abstract

We analyzed almost complete 18S rDNA sequences of 10 bovine Eimeria species, namely Eimeria alabamensis, E. auburnensis, E. bovis, E. bukidnonensis, E. canadensis, E. cylindrica, E. ellipsoidalis, E. subspherica, E. wyomingensis and E. zuernii. Although these sequences showed intraspecific variation in 8 species, the sequences of each species were clustered in monophyletic groups in all species, except E. auburnensis. The sequences constituted 3 distinct clusters in a phylogenetic tree with relatively high bootstrap values; however, the members including each cluster shared no similarities in oocyst morphology.

摘要

我们分析了10种牛艾美耳球虫的近乎完整的18S核糖体DNA序列，即阿拉巴马艾美耳球虫、奥本艾美耳球虫、牛艾美耳球虫、布基农艾美耳球虫、加拿大艾美耳球虫、柱状艾美耳球虫、椭圆艾美耳球虫、亚球形艾美耳球虫、怀俄明艾美耳球虫和邱氏艾美耳球虫。尽管这些序列在8个物种中显示出种内变异，但除奥本艾美耳球虫外，每个物种的序列在所有物种中都聚为单系群。这些序列在具有相对较高自展值的系统发育树中构成了3个不同的簇；然而，包括每个簇的成员在卵囊形态上没有相似之处。