用于检测粪便标本中腺病毒的酶联免疫吸附测定：与电子显微镜检查和病毒分离的比较
Enzyme linked immunosorbent assay for detecting adenoviruses in stool specimens: comparison with electron microscopy and isolation.
作者信息
Martin A L, Kudesia G
机构信息
Regional Virus Laboratory, Ruchill Hospital, Glasgow.
出版信息
J Clin Pathol. 1990 Jun;43(6):514-5. doi: 10.1136/jcp.43.6.514.
A commercial enzyme linked immunosorbent assay (ELISA) for detection of adenoviruses in stool samples was compared with the use of electron microscopy and isolation in Graham 293 cells. Although specific, the ELISA was less sensitive than both electron microscopy and isolation. The ELISA had an overall sensitivity of 78% and a specificity of 100%. The sensitivity was related to the amount of virus particles present in the stool sample, increasing to 90% with about 10(7) viral particles/ml of stool. The ELISA was easy to perform, requiring no instrumentation, and is a useful first line test for detection of adenoviruses in stool samples, especially in laboratories without access to an electron microscope. Wider use of ELISAs should help in evaluating the role of adenoviruses in viral gastroenteritis.
将一种用于检测粪便样本中腺病毒的商业酶联免疫吸附测定（ELISA）与电子显微镜检查以及在格雷厄姆293细胞中进行病毒分离的方法进行了比较。尽管ELISA具有特异性，但它的敏感性低于电子显微镜检查和病毒分离。ELISA的总体敏感性为78%，特异性为100%。敏感性与粪便样本中存在的病毒颗粒数量有关，当粪便中病毒颗粒约为10⁷个/毫升时，敏感性增至90%。ELISA操作简便，无需仪器设备，是检测粪便样本中腺病毒的一种有用的一线检测方法，尤其适用于没有电子显微镜的实验室。更广泛地使用ELISA有助于评估腺病毒在病毒性胃肠炎中的作用。
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