三种检测轮状病毒方法的敏感性评估。
An assessment of the sensitivity of three methods for the detection of rotavirus.
作者信息
Martin A L, Follett E A
出版信息
J Virol Methods. 1987 May;16(1-2):39-44. doi: 10.1016/0166-0934(87)90029-2.
PMID:3038941
Abstract
A comparison was made of the sensitivity of a current commercial ELISA for detecting rotavirus in faecal specimens with the more complex, technically demanding systems of electron microscopy and polyacrylamide gel electrophoresis. Even after modification, the ELISA failed to detect 22% of specimens with particles identifiable by electron microscopy. Polyacrylamide gel electrophoresis failed to identify 2 out of 50 specimens with particles present but did distinguish 2 group C rotaviruses.
摘要
将当前用于检测粪便标本中轮状病毒的商用酶联免疫吸附测定（ELISA）的灵敏度，与更复杂、技术要求更高的电子显微镜和聚丙烯酰胺凝胶电泳系统进行了比较。即使经过改进，ELISA仍未能检测出22%经电子显微镜鉴定有病毒颗粒的标本。聚丙烯酰胺凝胶电泳未能鉴定出50份有病毒颗粒的标本中的2份，但确实鉴别出了2株C组轮状病毒。
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