Wilson K H, Kennedy M J, Fekety F R
J Clin Microbiol. 1982 Mar;15(3):443-6. doi: 10.1128/jcm.15.3.443-446.1982.
Isolation of Clostridium difficile from fecal specimens has been facilitated by the development of a selective and differential medium, cefoxitin-cycloserinefructose agar (CCFA). We substituted 0.1% sodium taurocholate for the 2.5% egg yolk in CCFA and compared the growth of 15 isolates of C. difficile on the resulting medium with growth on conventional CCFA. The taurocholate-containing medium (TCCFA) quantitatively recovered vegetative forms of C. difficile in the same numbers as CCFA medium. Recovery of spores was a mean 1.7 log(10) higher on TCCFA than on CCFA. Thirty-six of 60 patient stool specimens growing C. difficile gave a heavier growth on TCCFA than on CCFA, and 9 failed to yield C. difficile on CCFA. TCCFA detected spores of 75 colony-forming units per ml from artificially inoculated fecal specimens when conventional stool culturing techniques were used. Fluorescence of colonies of C. difficile was more intense on TCCFA than on CCFA. TCCFA was simpler to prepare and, overall, was more sensitive than CCFA.
一种选择性鉴别培养基——头孢西丁-环丝氨酸-果糖琼脂（CCFA）的开发，促进了从粪便标本中分离艰难梭菌。我们用0.1%的牛磺胆酸钠替代了CCFA中2.5%的蛋黄，并将15株艰难梭菌在所得培养基上的生长情况与在传统CCFA上的生长情况进行了比较。含牛磺胆酸钠的培养基（TCCFA）回收的艰难梭菌营养体数量与CCFA培养基相同。TCCFA上孢子的回收率比CCFA平均高1.7个对数（10）。60份培养出艰难梭菌的患者粪便标本中，有36份在TCCFA上的生长比在CCFA上更旺盛，9份在CCFA上未培养出艰难梭菌。当使用传统粪便培养技术时，TCCFA能从人工接种的粪便标本中检测出每毫升75个菌落形成单位的孢子。艰难梭菌菌落在TCCFA上的荧光比在CCFA上更强。TCCFA制备更简单，总体而言比CCFA更敏感。
