School of Pathology and Laboratory Medicine, The University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, Australia.
Pathology. 2012 Jun;44(4):354-6. doi: 10.1097/PAT.0b013e328353a235.
Culture remains important for the detection and typing of Clostridium difficile. Culture of C. difficile spores can be enhanced on media supplemented with a germinant. Despite this, unsupplemented media continues to be used in some laboratories. The aim of this study was to quantify the effect of the known germinant sodium taurocholate on recovery of C. difficile spores and to determine if the supplement impacts on the recovery of vegetative C. difficile.
The recovery on cycloserine-cefoxitin-fructose agar (CCFA) with and without taurocholate, of spore, vegetative, and total cell fractions of broth cultures of eight C. difficile isolates was compared.
Taurocholate in CCFA did not inhibit growth of vegetative C. difficile and significantly increased recovery of spores (p = 0.04).
The routine incorporation of taurocholate in CCFA is recommended for improved sensitivity in C. difficile culture from specimens.
文化对于艰难梭菌的检测和分型仍然很重要。艰难梭菌孢子的培养可以通过添加发芽剂来增强。尽管如此，一些实验室仍在继续使用未添加补充剂的培养基。本研究的目的是量化已知发芽剂牛磺胆酸钠对艰难梭菌孢子回收的影响，并确定该补充剂是否会影响艰难梭菌的营养体回收。
比较了 8 株艰难梭菌分离株的菌液培养物的孢子、营养体和总细胞部分在添加和不添加牛磺胆酸钠的环丝氨酸头孢西丁果糖琼脂（CCFA）上的回收情况。
CCFA 中的牛磺胆酸钠不会抑制营养体艰难梭菌的生长，并且显著增加了孢子的回收（p=0.04）。
建议在 CCFA 中常规添加牛磺胆酸钠，以提高从标本中培养艰难梭菌的灵敏度。
