Upadhyay Harish C, Verma Ram K, Srivastava Santosh K
Medicinal Chemistry Department, Central Institute of Medicinal and Aromatic Plants, P.O.CIMAP, Lucknow–226015, India.
J Chromatogr Sci. 2013 Jan;51(1):21-5. doi: 10.1093/chromsci/bms099. Epub 2012 Jun 13.
Ammannia baccifera is an important component of various Chinese herbal formulations for which a rapid, simple, sensitive, gradient and reproducible reversed-phase high-performance liquid chromatographic method has been developed for the quantitative estimation of its bioactive constituents, 4-hydroxy-α-tetralone (4H), tetralone-4-O-β-D-glucopyranoside (T4) and ellagic acid (EA). The chromatographic separation of samples was performed on a Chromatopak Peerless C18 (250 × 4.6 mm i.d., 5 µm) column by gradient elution with 0.1% trifluoroacetic acid in water and methanol at a flow rate of 0.6 mL/min, a column temperature at 25°C and ultraviolet detection at λ 254 nm. The limit of detection (LOD) and limit of quantification (LOQ) were 1.51 and 5.06 µg/mL for EA, 0.70 and 2.33 µg/mL for T4 and 0.22 and 0.73 µg/mL for 4H, respectively. Good results were achieved with respect to linearity (r(2) > 0.999), repeatability (relative standard deviation ≤ 1.73%) and recovery (99.06-100.76%). The method was validated for linearity, accuracy, repeatability, LOQ and LOD. The method is simple, accurate and precise and was successfully applied to the analysis of these three analytes in five different leaf and root samples of A. baccifera; the method may be recommended for routine quality control analysis of various Chinese herbal formulations containing A. baccifera.
节节菜是多种中药配方中的重要成分，为此开发了一种快速、简便、灵敏、梯度洗脱且可重现的反相高效液相色谱法，用于定量测定其生物活性成分4-羟基-α-四氢萘酮（4H）、四氢萘酮-4-O-β-D-吡喃葡萄糖苷（T4）和鞣花酸（EA）。样品的色谱分离在Chromatopak Peerless C18（250×4.6 mm内径，5 µm）柱上进行，采用0.1%三氟乙酸水溶液和甲醇梯度洗脱，流速为0.6 mL/min，柱温25°C，紫外检测波长为λ 254 nm。EA的检测限（LOD）和定量限（LOQ）分别为1.51和5.06 µg/mL，T4为0.70和2.33 µg/mL，4H为0.22和0.73 µg/mL。在线性（r(2)>0.999）、重复性（相对标准偏差≤1.73%）和回收率（99.06 - 100.76%）方面取得了良好结果。该方法在线性、准确性、重复性、LOQ和LOD方面得到了验证。该方法简单、准确、精密，已成功应用于节节菜五个不同叶和根样品中这三种分析物的分析；该方法可推荐用于含节节菜的各种中药配方的常规质量控制分析。
