Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
J Nat Med. 2014 Jan;68(1):181-5. doi: 10.1007/s11418-013-0790-z. Epub 2013 Jun 27.
Asini Corii Collas (ACC; donkey glue) is a crude drug used to promote hematopoiesis and arrest bleeding. Because adulteration of the drug with substances from other animals such as horses, cattle, and pigs has been found, we examined PCR methods based on the sequence of the cytochrome b gene for source species identification. Two strategies for extracting DNA from ACC were compared, and the ion-exchange resin procedure was revealed to be more suitable than the silica-based one. Using DNA extracted from ACC by the ion-exchange resin procedure, PCR methods for species-specific detection of donkey, horse, cattle, and pig substances were established. When these species-specific PCR methods were applied to ACC, amplicons were obtained only by the donkey-specific PCR. Cattle-specific PCR detected as little as 0.1% admixture of cattle glue in the ACC. These results suggest that the species-specific PCR methods established in this study would be useful for simple and easy detection of adulteration of ACC.
阿胶（ACC；驴皮胶）是一种用于促进造血和止血的粗制药物。由于发现该药物与马、牛和猪等其他动物的物质混合，我们检查了基于细胞色素 b 基因序列的 PCR 方法来鉴定来源物种。比较了两种从 ACC 提取 DNA 的方法，发现离子交换树脂法比基于硅胶的方法更适合。使用离子交换树脂法从 ACC 中提取的 DNA，建立了驴、马、牛和猪物质的种特异性检测 PCR 方法。当将这些种特异性 PCR 方法应用于 ACC 时，仅通过驴特异性 PCR 获得了扩增子。牛特异性 PCR 检测到的 ACC 中牛胶的混合物低至 0.1%。这些结果表明，本研究中建立的种特异性 PCR 方法将有助于简单、轻松地检测 ACC 的掺假。
