Peter Neethumol, Chiramel Kevin J, A R Shivashankara
Lecturer in Biochemistry, Presentation College , Puthenvellikara, Kerala .
J Clin Diagn Res. 2013 May;7(5):797-800. doi: 10.7860/JCDR/2013/4658.2942. Epub 2013 May 1.
Alcohol biomarkers help in the early detection of alcoholism and its complications. There is a paucity of studies in India on the salivary markers of systemic diseases in general and on salivary alcohol biomarkers in particular.
The present study was aimed at assessing the effect of alcohol withdrawal on the antioxidants and amylase in blood and saliva, and at finding the correlation between the blood and the salivary parameters in alcoholics.
Sixty alcohol-dependent males who were in the age group of 30 - 70 years, who were admitted to the Deaddiction Centre for alcohol withdrawal treatment for one month, were the subjects of this study; age-matched healthy individuals were the controls. In the blood and saliva samples, the activities of Glutathione S-Transferase (GST) and amylase and the Total Antioxidant Capacity (TAC) were assayed.
The alcohol-dependent subjects showed significantly lower GST and amylase activities and the TAC in blood and saliva as compared to those in the controls (P<0.001). The alcohol withdrawal caused a significant increase in the GST and amylase activities and the TAC to near-control values. In the alcohol-dependent subjects, there was a significant correlation between the values in blood and saliva with respect to GST and TAC.
Alcoholism causes an impaired antioxidant capacity and a decreased secretion of amylase, which is ameliorated due to the alcohol withdrawal regimen . The strong correlation between blood and saliva with respect to the antioxidants suggests the potential future use of saliva as a laboratory tool in clinical medicine.
酒精生物标志物有助于酒精中毒及其并发症的早期检测。在印度，关于一般系统性疾病的唾液标志物，特别是唾液酒精生物标志物的研究较少。
本研究旨在评估戒酒对血液和唾液中抗氧化剂及淀粉酶的影响，并找出酗酒者血液和唾液参数之间的相关性。
本研究的对象为60名年龄在30至70岁之间、因戒酒治疗而入住戒毒中心一个月的酒精依赖男性；年龄匹配的健康个体作为对照。对血液和唾液样本中谷胱甘肽S-转移酶（GST）、淀粉酶的活性以及总抗氧化能力（TAC）进行了检测。
与对照组相比，酒精依赖者血液和唾液中的GST、淀粉酶活性以及TAC显著降低（P<0.001）。戒酒使GST、淀粉酶活性以及TAC显著增加至接近对照值。在酒精依赖者中，血液和唾液中GST及TAC的值之间存在显著相关性。
酗酒会导致抗氧化能力受损和淀粉酶分泌减少，而戒酒方案可改善这种情况。血液和唾液中抗氧化剂之间的强相关性表明唾液在未来临床医学中作为实验室工具的潜在用途。
