Banta Andreea, Bratosin Felix, Golu Ioana, Toma Ana-Olivia, Domuta Eugenia Maria
Doctoral School, Department of General Medicine, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.
Department of Infectious Disease, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.
Diagnostics (Basel). 2025 Feb 6;15(3):394. doi: 10.3390/diagnostics15030394.
Laryngeal squamous cell carcinoma (LSCC) is a common head and neck cancer with significant morbidity and mortality. Circulating microRNAs (miRNAs) have emerged as promising non-invasive biomarkers for cancer diagnosis and prognosis. This systematic review aims to identify circulating miRNAs associated with LSCC, emphasizing those validated by at least two independent studies to improve reliability and clinical applicability. An extensive literature search was performed in the PubMed, Scopus, and Web of Science databases up to October 2024, using keywords related to LSCC and circulating miRNAs. Studies involving human participants that provided quantitative data on circulating miRNA expression levels and their clinical correlations were included. Data extraction and quality assessment were conducted following standardized protocols, highlighting miRNAs reported in multiple studies. Nine high-quality studies encompassing 660 patients with LSCC and 212 controls were included. Several miRNAs were consistently identified across these studies. miR-21-5p was upregulated in four studies and associated with advanced disease stages, lymph node metastasis, and decreased survival rates. miR-125b-5p and miR-126-3p were consistently downregulated, linked to advanced clinical stages and poor tumor differentiation. miR-27a-3p was upregulated in two studies and correlated with poor prognosis, promoting LSCC progression by targeting Smad4. Additionally, miR-33a-5p was identified as a potential diagnostic biomarker with high sensitivity and specificity. These miRNAs show potential as non-invasive biomarkers for the diagnosis and prognosis of LSCC. This systematic review highlights specific circulating miRNAs-particularly miR-21-5p, miR-125b-5p, miR-126-3p, miR-27a-3p, and miR-33a-5p-as promising biomarkers for LSCC. The consistent findings across independent studies emphasize their potential clinical utility in early detection, prognostic assessment, and therapeutic targeting. However, further validation in larger and more diverse populations, along with the standardization of detection methods, is necessary before these biomarkers can be implemented in clinical practice.
喉鳞状细胞癌（LSCC）是一种常见的头颈癌，具有较高的发病率和死亡率。循环微RNA（miRNA）已成为癌症诊断和预后有前景的非侵入性生物标志物。本系统评价旨在识别与LSCC相关的循环miRNA，重点关注那些经过至少两项独立研究验证的miRNA，以提高可靠性和临床适用性。截至2024年10月，在PubMed、Scopus和Web of Science数据库中进行了广泛的文献检索，使用了与LSCC和循环miRNA相关的关键词。纳入了涉及人类参与者且提供了循环miRNA表达水平及其临床相关性定量数据的研究。按照标准化方案进行数据提取和质量评估，重点关注多项研究中报道的miRNA。纳入了9项高质量研究，共660例LSCC患者和212例对照。这些研究一致鉴定出了几种miRNA。miR-21-5p在4项研究中上调，与疾病晚期、淋巴结转移及生存率降低相关。miR-125b-5p和miR-126-3p持续下调，与临床晚期和肿瘤低分化相关。miR-27a-3p在2项研究中上调，与预后不良相关，通过靶向Smad4促进LSCC进展。此外，miR-33a-5p被鉴定为具有高灵敏度和特异性的潜在诊断生物标志物。这些miRNA显示出作为LSCC诊断和预后非侵入性生物标志物的潜力。本系统评价突出了特定的循环miRNA，特别是miR-21-5p、miR-125b-5p、miR-12
